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Rice grain shape regulation protein HOS59 as well as coding gene and application thereof

A technology that encodes genes and proteins, and is used in applications, genetic engineering, plant genetic improvement, etc.

Pending Publication Date: 2022-05-20
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, rice grain type is a complex regulatory process. If we want to comprehensively and systematically explain the regulatory mechanism of rice grain type, we still need to dig and identify more functional genes.

Method used

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  • Rice grain shape regulation protein HOS59 as well as coding gene and application thereof
  • Rice grain shape regulation protein HOS59 as well as coding gene and application thereof
  • Rice grain shape regulation protein HOS59 as well as coding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0097] Example 1. Regulation of Rice Grain Width and Grain Length by Controlling HOS59 Gene Level in Rice

[0098] 1. Construction of CRISPR-Cas9 recombinant plasmid

[0099] Rice KNOX family KNOXII subclass protein HOS59 consists of 317 amino acid residues, and its amino acid sequence is shown in sequence 2 in the sequence listing. The full-length CDS sequence of the HOS59 gene is shown as sequence 1 in the sequence listing.

[0100] In the CDS region of the HOS59 gene, search for the adjacent motif of the pre-spacer sequence, and design two guide RNA sequences gRNA1 and gRNA2 targeting the HOS59 gene. The nucleotide sequence of the gRNA1 target (HOS59-target 1) is 5'-GGACAAGGCTCGCTTGGTGC -3', corresponding to the 801-820th position of sequence 1 in the sequence listing; the nucleotide sequence of gRNA21 target (HOS59-target 2) is 5'-GGAAGAGCTGGAAAACTCCC-3', corresponding to sequence 1 in the sequence listing Nos. 711-730.

[0101] The primer pair HOS59-T1F / HOS59-T1R and t...

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Abstract

The invention discloses a rice grain shape regulation gene protein HOS59 as well as a coding gene and application thereof. One technical scheme protected by the invention is application of protein or a substance for regulating and controlling the activity or content of the protein in regulating and controlling or increasing the grain length of plant grains and / or the grain epidermal cell length. Experiments prove that after the expression quantity and / or activity of the HOS59 protein in the rice are / is reduced, the grain length and the grain epidermis cells of the obtained transgenic rice are obviously longer than those of wild rice Nipponbare; and in the HOS59 transgenic overexpression strain, the grain length and the grain epidermis cells are obviously shortened compared with those of the wild type. The research on the HOS59 is helpful for increasing new gene resources in the aspect of grain shape regulation and control of the plants; in actual production, rice grain traits can be regulated and controlled by regulating and controlling the HOS59, so that a new rice high-yield variety is cultivated.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to rice grain shape regulation protein HOS59 and its coding gene and application. Background technique [0002] Rice is one of the most important food crops in the world, once feeding more than half of the world's population. Rice is facing the real problem of increasing demand and decreasing arable land, which puts forward great requirements for increasing the yield of crops. In addition, according to forecasts, the world's major food crops rice, wheat and maize are expected to suffer substantial production losses due to climate change. This also puts forward high requirements for the environmental adaptability of rice. Under this requirement, the excavation of rice high-yield genes is of great significance for realizing the goal of "high yield, high quality, high efficiency, ecology, and safety" in rice production. [0003] Rice grain shape is an important indicator of rice yield a...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/84C12N15/82A01H5/00A01H6/46
CPCC07K14/415C12N15/8205C12N15/8261Y02A40/146
Inventor 苏震徐文英盛铭浩宋倩刘凤霞张群连李中秋曹亚欣薛天玺
Owner CHINA AGRI UNIV
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