Rice grain shape regulation protein HOS59 as well as coding gene and application thereof
A technology that encodes genes and proteins, and is used in applications, genetic engineering, plant genetic improvement, etc.
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[0097] Example 1. Regulation of Rice Grain Width and Grain Length by Controlling HOS59 Gene Level in Rice
[0098] 1. Construction of CRISPR-Cas9 recombinant plasmid
[0099] Rice KNOX family KNOXII subclass protein HOS59 consists of 317 amino acid residues, and its amino acid sequence is shown in sequence 2 in the sequence listing. The full-length CDS sequence of the HOS59 gene is shown as sequence 1 in the sequence listing.
[0100] In the CDS region of the HOS59 gene, search for the adjacent motif of the pre-spacer sequence, and design two guide RNA sequences gRNA1 and gRNA2 targeting the HOS59 gene. The nucleotide sequence of the gRNA1 target (HOS59-target 1) is 5'-GGACAAGGCTCGCTTGGTGC -3', corresponding to the 801-820th position of sequence 1 in the sequence listing; the nucleotide sequence of gRNA21 target (HOS59-target 2) is 5'-GGAAGAGCTGGAAAACTCCC-3', corresponding to sequence 1 in the sequence listing Nos. 711-730.
[0101] The primer pair HOS59-T1F / HOS59-T1R and t...
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