Single chromosome segregation method, single chromosome high-throughput sequencing library construction method and application

Abstract

The invention provides a single chromosome segregation method, a single chromosome high-throughput sequencing library construction method and application. According to the technical scheme, optimal dilution parameters are obtained through computer simulation experiments, chromosomes in single cell are segregated by a dilution method to obtain single chromosomes, and high-throughput sequencing and bioinformatics analysis are conducted so as to judge out that mutant genes derive from the father or the mother in homologous chromosomes. Compared with that the homologous chromosomes serve as a mixed sample for sequencing, the single chromosome segregation method has the advantages that the single chromosomes are segregated by the dilution method, and the single chromosome segregation method can be used for haplotype sequencing and has important application value in scientific research and clinical application.

Description

technical field [0001] The invention relates to the field of high-throughput sequencing, in particular to a single-staining separation method, a construction method and application of a single-chromosome high-throughput sequencing library. Background technique [0002] Human genome analysis efforts have rapidly moved from determining the "average" reference sequence of all people to the era of individual genome sequencing. At present, single cells have become the focus of scientists' attention, but the study of single chromosomes is still a blank field. Each cell in the human body contains two sets of genomes, one from the father and the other from the mother. This is called the haplotype phenomenon, and the variation in each haploid genome will affect the gene. Expression, protein function, and disease have a very large impact. [0003] At present, single-cell sequencing is relatively difficult. It is difficult to isolate a single chromosome from a single cell and build a...

AI Technical Summary

Problems solved by technology

At present, single cells have become the focus of scientists' attention, but the study of single chromosomes is still a blank field

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