Single chromosome segregation method, single chromosome high-throughput sequencing library construction method and application
A separation method and single-chromosome technology, applied in the field of high-throughput sequencing, can solve problems such as single chromosome blank, and achieve the effect of simple technical scheme and wide application range
Inactive Publication Date: 2015-11-25
SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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At present, single cells have become the focus of scientists' attention, but the study of single chromosomes is still a blank field
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[0111] The following description is the preferred embodiment of the present invention. It should be pointed out that for those skilled in the art, without departing from the principle of the present invention, several improvements and modifications can also be made, and these improvements and modifications are also regarded as It is the protection scope of the present invention.
[0112] Unless otherwise specified in the examples of the present invention, the reagents and consumables used are all commercially available commodities.
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The invention provides a single chromosome segregation method, a single chromosome high-throughput sequencing library construction method and application. According to the technical scheme, optimal dilution parameters are obtained through computer simulation experiments, chromosomes in single cell are segregated by a dilution method to obtain single chromosomes, and high-throughput sequencing and bioinformatics analysis are conducted so as to judge out that mutant genes derive from the father or the mother in homologous chromosomes. Compared with that the homologous chromosomes serve as a mixed sample for sequencing, the single chromosome segregation method has the advantages that the single chromosomes are segregated by the dilution method, and the single chromosome segregation method can be used for haplotype sequencing and has important application value in scientific research and clinical application.
Description
technical field [0001] The invention relates to the field of high-throughput sequencing, in particular to a single-staining separation method, a construction method and application of a single-chromosome high-throughput sequencing library. Background technique [0002] Human genome analysis efforts have rapidly moved from determining the "average" reference sequence of all people to the era of individual genome sequencing. At present, single cells have become the focus of scientists' attention, but the study of single chromosomes is still a blank field. Each cell in the human body contains two sets of genomes, one from the father and the other from the mother. This is called the haplotype phenomenon, and the variation in each haploid genome will affect the gene. Expression, protein function, and disease have a very large impact. [0003] At present, single-cell sequencing is relatively difficult. It is difficult to isolate a single chromosome from a single cell and build a...
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IPC IPC(8): C12N5/078C40B50/06C12Q1/68
Inventor 贺建奎张萌罗登郭佳杰王嫣陈杳
Owner SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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