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Orychophragmus violaceus chromosome acetic acid orcein banding method

A technology of lichen red and chromosomes, applied in the field of cytogenetics, to achieve the effect of simplified procedure, high success rate of banding and good dispersion degree

Inactive Publication Date: 2012-01-25
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0018] The above-mentioned method of collecting materials for small chromosomes of Zhuge Cai cannot meet the banding effect, and the success rate of banding is low. Colchicine is used for pretreatment, which is toxic and pollutes the environment
After dissociation, the tablet is pressed, and then the banding treatment is carried out before the banding can be dyed. There are many operation steps, the uncertainty increases, and the success rate of banding is low.

Method used

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Experimental program
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Effect test

Embodiment Construction

[0044] 1) Take materials. Soak the seeds in warm water for 8 hours, then put them in a 600mg / L gibberellin solution and put them in a refrigerator at 4°C for 24 hours. The seeds were then germinated in a petri dish at 22°C, and placed on filter paper moistened with distilled water, protected from light. When the seeds germinated, put the seeds in the refrigerator at 4°C for 24 hours, then take them out and put them back in the greenhouse at 22°C. When the root grows to 1-2 cm, the root tip meristem is intercepted. 2) Pretreatment. After the plant root tip meristem was treated with ice water at 0-1°C for 23h, the material was fixed at 4°C for about 24h with Carnot's solution I (absolute alcohol: glacial acetic acid = 3:1). 3) Dissociation. The material was dissociated in 1mol / L HCl preheated at 60°C, and the dissociation time was eight minutes. 4) Dyeing. Put the material into 0.1% lichenin acetate staining solution by mass percentage and dye at room temperature for 24 ho...

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Abstract

The invention discloses an orychophragmus violaceus chromosome acetic acid orcein banding method for solving the problems that the traditional chromosome separation banding method is not suitable for small chromosome plants such as orychophragmus violaceus, the procedure is complex, the operating conditions are not easily mastered and the banding success rate is low. Cell chromosomes can be easily separated by adopting acetic acid orcein for dyeing and combining a chromosome preparation method, the chromosomes have good shape after being lightly pressed and are clear in banding display, and identification of target chromosomes is facilitated. The plant chromosome banding method is very simple and convenient and easy in operation, is particularly suitable for banding pattern analysis of plants with small chromosomes, and is clear in displayed banding patterns, high in success rate and convenient for genetic analysis of plants.

Description

Technical field: [0001] The invention belongs to the field of cytogenetics, and in particular relates to a method for developing chromosome banding of Zhuge cuisine. Background technique: [0002] Chromosome banding is a routine and classic technique for identifying chromosomes and studying chromosome structural variation. It is to process the chromosomes through a certain program and dye them with specific dyes, so that the chromosomes show horizontal stripes of alternating light and dark or different shades on the long axis of the chromosomes. Such stripes are called chromosome bands. Banding technology can reveal the "anatomical" characteristics of chromosomes, can display the specific banding pattern of each chromosome in eukaryotes, and provide a wealth of special markers that can be used for identification of chromosomes, which provides the necessary for identification and analysis of each chromosome conditions, and thus can identify chromosomes more accurately than...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 周丽蓉陈文清冯雷
Owner SICHUAN UNIV
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