The invention discloses a formula of novel environment-friendly biological hydrocarbon oil and a preparation method of the novel environment-friendly biological hydrocarbon oil. The novel environment-friendly biological hydrocarbon oil is prepared by mixing the following raw materials: 1-1000kg of polyester-grade diethylglycerol, tap water, 0.5-500kg of purified water and a catalyst, no black smoke and no peculiar smell are discharged, no waste is discharged in the production process, no pollution is caused to the environment, and no harm is caused to human health; the oil is high in calorific value and low in price, all indexes of the oil meet national standards through detection, the oil can replace diesel oil to become new energy of enterprises, the oil is high in flash point, limit 1.9%, high in ignition point, free of open fire, free of fire, safe to use and wide in raw material source, waste is utilized, waste is turned into wealth, ecological resources are protected, and the oil belongs to renewable energy and is worthy of popularization. The method has good economic benefits and social benefits, and has good popularization value.