Molecular markers for identification of chromosome segregation of a03 and c03 in hybrids and progeny materials of Chinese cabbage and Ethiopian mustard

A molecular marker and chromosome technology, applied in the field of genetics and breeding, can solve problems such as time-consuming and labor-intensive, and achieve the effect of simple and fast cost, enriching vegetable types, and reducing labor.

Active Publication Date: 2022-04-22
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Description
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AI Technical Summary

Problems solved by technology

Due to the reproductive isolation of species, distant hybridization often requires techniques such as artificial pollination and embryo rescue, which is time-consuming and laborious, and requires certain scientific training

Method used

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  • Molecular markers for identification of chromosome segregation of a03 and c03 in hybrids and progeny materials of Chinese cabbage and Ethiopian mustard
  • Molecular markers for identification of chromosome segregation of a03 and c03 in hybrids and progeny materials of Chinese cabbage and Ethiopian mustard
  • Molecular markers for identification of chromosome segregation of a03 and c03 in hybrids and progeny materials of Chinese cabbage and Ethiopian mustard

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 This example identifies the hybrid F between Chinese cabbage and Ethiopian mustard 1 plant

[0041] 1.1 Extract the F to be detected 1 Genomic DNA of the plant and its parents.

[0042] 1.2 Synthetic primers:

[0043] C03B2-F: 5'-CCTCCGCTAAGGTAAATCTCG-3' (SEQ ID No.1);

[0044] C03B2-R: 5'-GCCGGAAGAAGAGATCAGTTT-3' (SEQ ID No. 2).

[0045] 1.3 PCR amplification. To be detected F 1 Plants and their parental DNA are used as templates, and the above primers are used for PCR amplification reaction. The reaction system is 10 μL, including: 1×PCR Buffer (containing Mg 2+ ), 1ng template DNA, 0.2mM dNTPs, 0.5μM primer C03B2-F, 0.5μM primer C03B2-R, 1U Taq enzyme. PCR reaction conditions: 94°C for 3min; 94°C for 30s, 58°C for 30S, 72°C for 30S, 35 cycles; 72°C for 5min.

[0046] 1.4 The above PCR products were detected by polyacrylamide gel electrophoresis. Configure 8% polypropylene gel, run electrophoresis at 180 volts for 1.5 hours, and end the electrophor...

Embodiment 2

[0053] Example 2 This example identifies the hybrid backcross between Chinese cabbage and Ethiopian mustard and then the selfed progeny (BC 1 f 2 )Material

[0054] 1.1 Extract the genomic DNA of the plants to be tested and their parents.

[0055] 1.2 Synthetic primers:

[0056] C03B2-F: 5'-CCTCCGCTAAGGTAAATCTCG-3' (SEQ ID No.1);

[0057] C03B2-R: 5'-GCCGGAAGAAGAGATCAGTTT-3' (SEQ ID No. 2).

[0058] 1.3 PCR amplification. Taking the plant to be detected and its parental DNA as a template, the PCR amplification reaction is carried out with the above primers. The reaction system is 15 μL, including: 1×PCR Buffer (containing Mg 2+ ), 1ng template DNA, 0.2mM dNTPs, 0.5μM primer C03B2-F, 0.5μM primer C03B2-R, 1U Taq enzyme. PCR reaction conditions: 95°C for 3min; 95°C for 30s, 58°C for 30S, 72°C for 30S, 35 cycles; 72°C for 10min.

[0059] 1.4 The above PCR products were detected by polyacrylamide gel electrophoresis. Configure 8% polypropylene gel, run electrophoresis at ...

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Abstract

The invention discloses a molecular marker and a method for identifying hybrids between Chinese cabbage and Ethiopian mustard and tracking the chromosomal separation of A03 and C03 offspring materials, belonging to the field of plant genetics and breeding. This marker is a pair of co-dominant molecular markers, which can be used to identify the authenticity of hybrids between Chinese cabbage and Ethiopian mustard, and can also be used to identify and track self-crossed offspring, backcrossed offspring, chromosome addition lines, and recombination of distant hybrids Chromosomal segregation of A03 and C03 in segregating populations and plants.

Description

technical field [0001] The invention relates to the field of genetic breeding, in particular to a method for identification and selection of distant hybrid plants. Background technique [0002] Distant hybridization is an important means to create new plant germplasm and expand breeding resources. Due to the reproductive isolation of species, distant hybridization often requires techniques such as artificial pollination and embryo rescue, which is time-consuming and laborious, and requires certain scientific training. In the process of distant hybridization, false hybrid plants may be produced due to incomplete castration, female gametes developing into plants, and other reasons. Therefore, the plants obtained by distant hybridization need to be tested whether they are true hybrids by molecular, cytological and other methods. The offspring of self-crossing and backcrossing of distant hybrids need to track chromosomes or chromosome fragments by molecular or cytological meth...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 宋江萍张晓辉李锡香王海平
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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