Method for screening mycosphaerella melonis resistance IL14 of cucumber-pickled cucumber introgression line

Abstract

The invention relates to a method for screening mycosphaerella melonis-resistant IL 14 of cucumber-pickled cucumber introgression line. According to batch treatment of different concentrations, plants with good resistance are screened, and the method belongs to the field of biotechnology breeding. The method comprises the following steps: by taking wild species pickled cucumber as a female parent, cultivating cucumber 'Beijing jietou' as a male parent, and hybridizing to obtain hybrid F1; combining the hybrid F1 with a somaclone variation chromosome reduplication technology by virtue of embryo rescue, thereby obtaining a fertile inter-specific heterologous tetraploid new species which is named as Cucumis hytivus; and by taking the heterologous tetraploid new species Cucumis hytivus as a female parent, performing system backcrossing and self-cross selective breeding with the cultivated variety 'Beijing jietou', thereby obtaining 86 parts of cucumber inter-specific introgression line materials carried with wild species chromosome segments, and respectively numbering as IL1-IL86. The 86 parts of introgression line groups are screened by adopting the greenhouse seedling stage artificial inoculation identification technology, stable moderate resistance or more strains are obtained by virtue of inoculation treatment in spring and autumn, the inoculation treatment is performed by increasing the concentration of bacterium, and the mycosphaerella melonis resistance strain IL14 is screened.

Description

technical field [0001] The invention discloses a method for screening cucumber-sour cucumber introgression lines resistant to vine blight IL14, which screens out plants with good resistance through batch processing at different concentrations, and belongs to the field of biotechnology breeding. Background technique [0002] Cucumber (Cucumis sativus L.) is one of the top ten vegetables in the world, mainly distributed in temperate and subtropical regions. According to the statistics of the Food and Agriculture Organization of the United Nations (FAO), the cultivated area of ​​cucumber in China in 2010 was 988 680hm 2 , with a total output of 40 710 200t, it is the world's largest producer and consumer of cucumbers. In recent years, with the continuous development of facility cucumbers, cucumber blight (Mycosphaerella melonis) has become the second major disease that endangers cucumber production after root-knot nematode. The disease occurs frequently in high-temperature an...

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Problems solved by technology

[0004] The genetic background of cucumber is narrow, and the variation rate is only 3-8% (Staub et al., 1987). Disease-resistant resources are relatively scarce, and it is difficult to obtain excellent disease-resistant resources or mutants under natural conditions. The process was once stuck in the bottleneck stage

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