New technology for extracting L-glutamine

Abstract

The invention belongs to the technical field of fermentation and discloses a new technology for extracting L-glutamine. The new technology for extracting the L-glutamine comprises the following steps: fermenting, carrying out micromembrane filtration, concentrating, crystallizing, carrying out ion exchange, recrystalizing, and preparing a fertilizer. The new technology for extracting the L-glutamine has the advantages that fermentation efficiency is high, an extraction technology is easy and feasible, environmental protection and no pollution are realized, and waste materials are changed into things of value.

Description

technical field

[0001] The invention relates to a new process for extracting L-glutamine, which belongs to the technical field of fermentation. Background technique

[0002] L-glutamine (L-Gln), also known as L-glutamine, is an amino acid that is amidated by the γ-carboxyl group of L-glutamic acid. It has many special functions and is classified as a conditionally essential amino acid. It is clinically used to treat diseases such as alcoholism, listlessness, and gastric and duodenal ulcers. In addition, it also has the functions of improving nervous function, improving memory impairment after cerebral hemorrhage, improving the body's immunity, promoting the intellectual development of children with mental retardation, and preventing epileptic seizures. It is currently widely used in clinical medicine, health food and animal feed industries. superior.

[0003] Japan was the first to use the fermentation method to produce L-glutamine. In 1977, the output of L-glutamine was 1...

Examples

Embodiment 1

[0031] A new process for extracting L-glutamine, which comprises the steps of:

[0032] 1) Corynebacterium glutamicum CCTCC NO.M205028 (see Chinese Patent 200510043704) and Brevibacterium flavum ATCC14067 (see "Selection of medium for producing L-glutamine with Brevibacterium flavum ATCC 14067", Amino Acid and Microbial Resources 1984 ) were cultured to a concentration of 1×10 8 Bacteria solution per mL, mixed according to the volume ratio of 3:1 to obtain the mixed bacterium solution, then inoculated into the fermentation medium according to 3% inoculum, and fermented and cultivated for 32 hours to obtain L-glutamine fermentation liquid; wherein The base is (mass percentage): glucose 12%, corn steep liquor 0.6%, MgSO 4 ·7H 2 O 0.3%, K 2 HPO 4 ·3H 2 O 0.2%, NaCl 0.2%, MnSO 4 0.001%, FeSO 4 0.001%, V B1 0.0001%, pH 7.0; The concentration of L-glutamine in the obtained L-glutamine fermentation broth is 95g / L;

[0033] 2) The L-glutamine fermentation liquid is filtere...

Embodiment 2

[0043] The fertilizer effect test of the compound fertilizer prepared by the present invention

[0044] 1. Fertilizer experiment for planting sorghum:

[0045] The control group adopts nitrogen, phosphorus and potassium compound fertilizer (nitrogen 16 phosphorus 22 potassium 7), and the experimental group adopts the compound fertilizer prepared by the embodiment of the present invention 1, and the soil quality and planting conditions of the test field are exactly the same, and the area is 10 mu; 50kg / mu; specific test results are shown in Table 1:

[0046] Table 1

[0047]

[0048] Note: the compound fertilizer is calculated at 2.8 yuan / kg, the organic fertilizer of the present invention is calculated at 2.4 yuan / kg, and the sorghum is calculated at 3.0 yuan / kg.

[0049] Second, planting sweet potato fertilizer experiment:

[0050] Set up two treatment test fields, both with an area of ​​10 mu, which are the control group and the experimental group. The control group a...

technical field

[0001] The invention relates to a new process for extracting L-glutamine, which belongs to the technical field of fermentation. Background technique

[0002] L-glutamine (L-Gln), also known as L-glutamine, is an amino acid that is amidated by the γ-carboxyl group of L-glutamic acid. It has many special functions and is classified as a conditionally essential amino acid. It is clinically used to treat diseases such as alcoholism, listlessness, and gastric and duodenal ulcers. In addition, it also has the functions of improving nervous function, improving memory impairment after cerebral hemorrhage, improving the body's immunity, promoting the intellectual development of children with mental retardation, and preventing epileptic seizures. It is currently widely used in clinical medicine, health food and animal feed industries. superior.

[0003] Japan was the first to use the fermentation method to produce L-glutamine. In 1977, the output of L-glutamine was 1...

Examples

Embodiment 1

[0031] A new process for extracting L-glutamine, which comprises the steps of:

[0032] 1) Corynebacterium glutamicum CCTCC NO.M205028 (see Chinese Patent 200510043704) and Brevibacterium flavum ATCC14067 (see "Selection of medium for producing L-glutamine with Brevibacterium flavum ATCC 14067", Amino Acid and Microbial Resources 1984 ) were cultured to a concentration of 1×10 8 Bacteria solution per mL, mixed according to the volume ratio of 3:1 to obtain the mixed bacterium solution, then inoculated into the fermentation medium according to 3% inoculum, and fermented and cultivated for 32 hours to obtain L-glutamine fermentation liquid; wherein The base is (mass percentage): glucose 12%, corn steep liquor 0.6%, MgSO 4 ·7H 2 O 0.3%, K 2 HPO 4 ·3H 2 O 0.2%, NaCl 0.2%, MnSO 4 0.001%, FeSO 4 0.001%, V B1 0.0001%, pH 7.0; The concentration of L-glutamine in the obtained L-glutamine fermentation broth is 95g / L;

[0033] 2) The L-glutamine fermentation liquid is filtere...

Embodiment 2

[0043] The fertilizer effect test of the compound fertilizer prepared by the present invention

[0044] 1. Fertilizer experiment for planting sorghum:

[0045] The control group adopts nitrogen, phosphorus and potassium compound fertilizer (nitrogen 16 phosphorus 22 potassium 7), and the experimental group adopts the compound fertilizer prepared by the embodiment of the present invention 1, and the soil quality and planting conditions of the test field are exactly the same, and the area is 10 mu; 50kg / mu; specific test results are shown in Table 1:

[0046] Table 1

[0047]

[0048] Note: the compound fertilizer is calculated at 2.8 yuan / kg, the organic fertilizer of the present invention is calculated at 2.4 yuan / kg, and the sorghum is calculated at 3.0 yuan / kg.

[0049] Second, planting sweet potato fertilizer experiment:

[0050] Set up two treatment test fields, both with an area of ​​10 mu, which are the control group and the experimental group. The control group a...

Images