Creation method of pumpkin interspecific crossing recombinant inbred line

An inbred line and pumpkin technology, which is applied in the field of vegetable genetics and breeding, can solve the problems of difficulty in obtaining and hybridization between pumpkin and Indian pumpkin, etc., and achieves the effect of strong operability.

Inactive Publication Date: 2018-04-24
ZHEJIANG UNIV
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  • Abstract
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Problems solved by technology

[0003] The technical problem to be solved by the present invention is to provide a method for creating a recombinant inbred line of pumpkin interspecific hybridization. The method

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  • Creation method of pumpkin interspecific crossing recombinant inbred line
  • Creation method of pumpkin interspecific crossing recombinant inbred line
  • Creation method of pumpkin interspecific crossing recombinant inbred line

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Experimental program
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Embodiment 1

[0022] Embodiment 1, the creation method of pumpkin interspecific hybridization recombinant inbred line, carry out the following steps successively:

[0023] 1 Materials and methods

[0024] 1.1 Test materials and sources:

[0025] Indian squash P1 and P2 are inbred lines. where P 1 It is a multi-generation inbred line selected from the Indian pumpkin variety Chestnut vine of Japan Kyowa Seedling Co., Ltd., P 2 It is a multi-generation inbred line selected from the Indian pumpkin variety Sheng Rong of Japan Musashino Seedling Co., Ltd. Chinese Pumpkin P 3 , is a multigenerational inbred line of Chinese pumpkin elephant trunk.

[0026] 1.2 Test method:

[0027] From the spring of 2006 to the autumn of 2009, two seasons of spring and autumn are planted every year, and a total of 8 crops are planted in four years. Among them, in the spring of 2006, Indian pumpkin P 1 ,P 2 As a parent, Chinese squash P. 3 Carry out reciprocal crosses for parents, and use Indian pumpkin P...

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Abstract

The invention discloses a creation method of a pumpkin interspecific crossing recombinant inbred line. The method comprises the steps that winter squash is used a female parent, cucurbita moschata isused as a male parent, F1 hybrid seeds are obtained, then winter squash is used as a recurrent parent, and the interspecific crossing recombinant inbred line is obtained through a backcross and selfing method; the interspecific crossing recombinant inbred line has the performance of progeny reproduction. Along with increasement of the selfing generation number, the progeny fertility is gradually restored, the fertility of high-generation inbred lines of the fourth inbred generation and the fifth inbred generation is basically close to a normal level, and the reproductive performance of the interspecific crossing recombinant inbred line is good. By adopting the method, the problems can be solved that hybridization of cucurbita moschata and winter squash is difficult, and interspecific crossing hybrids and selfed progeny thereof are difficultly obtained.

Description

technical field [0001] The invention belongs to the field of vegetable genetics and breeding, and specifically relates to a technical method suitable for creation of pumpkin interspecific hybridization recombination inbred lines. Background technique [0002] Pumpkin is an important economic crop of cucurbit vegetables, including Chinese pumpkin, Indian pumpkin, American pumpkin and so on. Chinese pumpkins have strong disease resistance and heat resistance, but they are not cold-resistant, late ripening, and tender melons are of poor quality. Generally, old and ripe melons are eaten, while Indian pumpkins are early-maturing and of good quality, but weak in disease resistance and poor in heat resistance. , generally based on edible tender melon. Therefore, the interspecific hybridization between Indian squash and Chinese squash is an important way to improve the traits of pumpkin resistance and quality. The interspecific hybridization between Indian squash and Chinese squas...

Claims

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Application Information

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IPC IPC(8): A01H1/02
CPCA01H1/02
Inventor 张明方叶飞华金永祥林万开
Owner ZHEJIANG UNIV
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