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Cultivation method of virus-resistant sweet potato interspecific introgression line

A breeding method and technology of introgression lines, applied in the field of cultivation of virus-resistant sweet potato interspecific introgression lines, can solve the problems of low fruit setting rate, failure to meet flowering, hybrid sterility, etc., and achieve the effect of broadening the genetic basis

Active Publication Date: 2018-07-24
XUZHOU INST OF AGRI SCI IN JIANGSU XUHUAI DISTRICT (JIANGSU XUZHOU SWEETPOTATO CENT)
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Problems solved by technology

[0009] The object of the present invention is to provide a method for cultivating highly efficient virus-resistant sweet potato interspecific introgression lines, solving the problems of poor flowering, low fruit setting rate and hybrid sterility

Method used

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  • Cultivation method of virus-resistant sweet potato interspecific introgression line
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  • Cultivation method of virus-resistant sweet potato interspecific introgression line

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Embodiment 1

[0023] It has the introgression line of I. violacea blood, and the specific cultivation method is as follows:

[0024] 1. Obtaining hybrids between sweet potato and related wild species

[0025] 1) Preparation of parent materials: 18 kinds of potatoes from the female parent Xushu were planted in the solar greenhouse in early April in spring and covered with plastic film. At the end of April, the wild species I. violacea and Japanese morning glory I. nil (rootstock) seeds were sown in 10×10 cm nutrient pots after soaking and accelerating germination. In mid-May, when the stem base of the rootstock is 0.5-1cm long, select robust plants to cut off the top for grafting, and cut healthy high-cut Xushu 18 seedlings with a diameter of about 0.5-1cm to make the scion. Grafting by grafting. After 1-2 weeks of grafting and surviving, move them into hybrid pots, and carry out 8h short-day photoperiod treatment. When wild species have two leaves and one heart (two true leaves are unfol...

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Abstract

The invention discloses a cultivation method of a virus-resistant sweet potato interspecific introgression line. The method comprises the following steps: performing interspecific hybridization on broad affinity parent Xu potatoes 18 and virus-resistant sweet potato wild species to obtain interspecific hybrid seedling seeds on the basis of guaranteeing the hybridization quantity and performing treatment by using hybridization treatment liquid; performing tracking identification on the obtained hybrid F1 group, selecting an SPVD-resistant true hybrid as a female parent and cultivated species asa recurrent parent, and performing continuous back cross for three generations and establishing a BC3F1 group; and performing resistance identification and labeling analysis on the BC3F1 group to obtain a virus-resistant introgression group BC3F1. The traditional genetic group construction method of the sweet potatoes is broken through, the sweet potato genetic basis is broadened, a high-densitygenetic map can be constructed by a small group scale, and manpower and material resource expenditures caused by application of too much marks are reduced. Meanwhile, the method can be applied to improvement of an anti-SPVD variety.

Description

technical field [0001] The invention belongs to the field of biotechnology breeding, and relates to a method for cultivating virus-resistant sweet potato interspecific introgression lines. Background technique [0002] The cultivated sweet potato Ipomoea batatas Lam L. (2n=6x=90) belongs to the genus Ipomoea genus Convolvulaceae. It is a starch-rich root crop with high yield, wide adaptability and many uses, because of its high starch content and alcohol conversion rate. It has become one of the main raw materials for the production of fuel ethanol in my country, and it is also an important raw material for feed, vegetable and starch processing, and has health functions such as anti-cancer, anti-diabetes, and anti-oxidation. [0003] Sweetpotato virus disease (Sweetpotato virus diseases, SPVD) is mainly caused by the interaction between sweetpotato plume mosaic virus (SPFMV) and sweetpotato chlorotic dwarf virus (SPCSV), in which SPCSV can increase the content of SPFMV virus ...

Claims

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Application Information

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IPC IPC(8): A01H1/02
CPCA01H1/02
Inventor 曹清河赵冬兰张安唐君周志林戴习彬
Owner XUZHOU INST OF AGRI SCI IN JIANGSU XUHUAI DISTRICT (JIANGSU XUZHOU SWEETPOTATO CENT)
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