Protein related to rice panicle type and encoding gene and application thereof
A technology that encodes genes and proteins, applied in applications, genetic engineering, plant genetic improvement, etc., can solve the problems of narrow genetic base and poor heterogeneity, and achieve the effect of thick stems, compact panicles, and straight leaves
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Embodiment 1
[0033] Embodiment 1, the isolation of gene
[0034] Erect panicle mutant japonica erect panicle 2 (jep2) source: the inventor of the present invention obtained the tissue cultured rice variety Yandao No. 8, and obtained the mutant on September 17, 2007.
[0035] The rice variety Yandao No. 8 is a conventional rice cultivar, which can be purchased from Yancheng Tomorrow Seed Industry Technology Co., Ltd., Jiangsu Province.
[0036] 1. Phenotype analysis of rice mutant jep2
[0037] (1) Phenotype analysis of rice mutant jep2
[0038] A rice erect panicle mutant named japonica erect panicle 2 (jep2) was screened from the tissue culture progeny of Yandao No. 8. The mutant has excellent agronomic traits such as thick stems and straight leaves. The main feature of this mutant is that the ear keeps upright after heading. The mutant plants were not significantly different from the wild type from vegetative growth to early flowering ( figure 1 A); But after grain filling, the pan...
Embodiment 2
[0066] Embodiment 2, the application of gene
[0067] 1. Functional complementation experiment of erect panicle mutant jep2
[0068] BAC Os JNBb0035M20 (Liu, X.H., Lu, T.T., Yu, S.L., Li, Y., Huang, Y.C., Huang, T., Zhang, L., Zhu, J.J., Zhao, Q., Fan, D.L., Mu, J., Shanghai, Y.Y., Feng, Q., Guan, J.P., Ying, K., Zhang, Y., Lin, Z.X., Sun, Z.X., Qian, Q., Lu, Y.P. & Han, B._A collection of 10 , 096 indica rice full-length cDNAs reveals highly expressed sequence divergence between Oryza sativa indica and japonica subspecies. Plant Mol Eiol., 65, 403-415 (2007).) (Institute of Genetics and Developmental Biology, Chinese Academy of Sciences).
[0069] Mutant jep2 can be provided by the Institute of Genetics and Developmental Biology, Chinese Academy of Sciences.
[0070] Agrobacterium tumefaciens (AgroBac terium tumefaciens) strain EHA105 was purchased from the Culture Collection Center of the Institute of Microbiology, Chinese Academy of Sciences.
[0071]BAC Os JNBb0035M20 w...
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