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Protein related to rice panicle type and encoding gene and application thereof
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A technology that encodes genes and proteins, applied in applications, genetic engineering, plant genetic improvement, etc., can solve the problems of narrow genetic base and poor heterogeneity, and achieve the effect of thick stems, compact panicles, and straight leaves
Active Publication Date: 2010-06-09
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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However, most of the erect panicle varieties currently planted are derived from the ancient Italian variety Barilla, with a narrow genetic base and poor heterogeneity, and there are inherent genetic links between erect panicle and some morphological and physiological traits
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Embodiment 1
[0033] Embodiment 1, the isolation of gene
[0034] Erect panicle mutantjaponica erect panicle 2 (jep2) source: the inventor of the present invention obtained the tissue cultured rice variety Yandao No. 8, and obtained the mutant on September 17, 2007.
[0035] The rice variety Yandao No. 8 is a conventional rice cultivar, which can be purchased from Yancheng Tomorrow Seed Industry Technology Co., Ltd., Jiangsu Province.
[0038] A rice erect panicle mutant named japonica erect panicle 2 (jep2) was screened from the tissue culture progeny of Yandao No. 8. The mutant has excellent agronomic traits such as thick stems and straight leaves. The main feature of this mutant is that the ear keeps upright after heading. The mutant plants were not significantly different from the wild type from vegetative growth to early flowering ( figure 1 A); But after grain filling, the pan...
Embodiment 2
[0066] Embodiment 2, the application of gene
[0067] 1. Functional complementation experiment of erect panicle mutant jep2
[0068] BAC Os JNBb0035M20 (Liu, X.H., Lu, T.T., Yu, S.L., Li, Y., Huang, Y.C., Huang, T., Zhang, L., Zhu, J.J., Zhao, Q., Fan, D.L., Mu, J., Shanghai, Y.Y., Feng, Q., Guan, J.P., Ying, K., Zhang, Y., Lin, Z.X., Sun, Z.X., Qian, Q., Lu, Y.P. & Han, B._A collection of 10 , 096 indica rice full-length cDNAs reveals highly expressed sequence divergence between Oryza sativa indica and japonicasubspecies. Plant Mol Eiol., 65, 403-415 (2007).) (Institute of Genetics and Developmental Biology, Chinese Academy of Sciences).
[0070] Agrobacterium tumefaciens (AgroBac terium tumefaciens) strain EHA105 was purchased from the Culture Collection Center of the Institute of Microbiology, Chinese Academy of Sciences.
[0071]BAC Os JNBb0035M20 w...
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Abstract
The invention discloses a protein related to rice panicle type and an encoding gene and an application thereof. The protein is the a) or b) protein as follows: a) the protein consisting of an amino acid sequence shown as the sequence 4 in a sequence table; and b) the protein derived by substituting and / or deleting and / or adding one or a plurality of amino acid residues of the amino acid sequence shown as the sequence 4 in the sequence table and being related to the plantpanicle type. Functional complementary experiments and transgenic experiments probe that the protein and the encoding gene thereof provided by the invention can control the plantpanicle type, in particular to promote plants to show the erect panicle phenotype; and the protein and the encoding gene thereof enhance the research of the breeding of erect panicle type rice, thereby having important significance for expanding the genetic basis of the types of the existing erect panicle type and playing a significant role in genetic breeding of the rice.
Description
technical field [0001] The invention relates to a rice panicle-type related protein, its coding gene and application. Background technique [0002] Rice is an important food crop in the world and the largest food crop in my country. The country's rice sown area accounts for 27.90% of the grain sown area, and its total output accounts for 39.82% of the total grain crop output. 60% of the population in my country feeds on rice. Therefore, rice production has an important strategic position in my country's food production. Since the 1960s, the use of the semi-dwarf gene sd1 and the cytoplasmic male sterility gene has achieved a second leap in rice yield. From high-stalk farm varieties to high-stalk improved varieties to short-stalk improved varieties, the yield has increased by about 20%-30%; from dwarf varieties to hybrid rice, the yield has increased by about 20%. However, rice yields have stagnated for quite a long time after these two big leaps. Plant architecture is a...
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