Multi-parental hybrid breeding method of tilapia
A technology for hybrid seed production and tilapia, which is applied in the field of multi-hybrid tilapia seed production, can solve the problems of low seedling quantity, unstable male rate, influence on the utilization of Neo's hybrid vigor and hybrid promotion, etc. High, high offspring male rate, the effect of improving offspring male rate
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Embodiment 1
[0023] 1 Materials and methods
[0024] 1.1 Test material
[0025] The experimental parents of tilapia were provided by the Weiye Tilapia Breeding Farm in Maoming City, Guangdong Province, including the new Jifu strain Nile tilapia (selected by Shanghai Ocean University F15), the Sudan strain Nile tilapia (bred from Sudan in 2008 Introduced from the confluence of the White Nile and the Blue Nile), the Maoming strain Olia tilapia (introduced by the Guangzhou Fisheries Research Institute in 1981) and the Israeli strain Olia tilapia (introduced from the Pearl River Fisheries Research Institute in 2003, derived from Israel). In July 2013, the intraspecific binary hybrid parents of Nile tilapia and Oria tilapia were prepared respectively, Nile tilapia: New Gifu♀×Sudan 、Sudan♀×Xin Jifu , Olia tilapia: Israel ♀×Maoming , Maoming♀×Israel . After emergence in August, they were cultivated in outdoor cement pools (5×9×1.5m), fed with compound feed for tilapia, with an average d...
Embodiment 2
[0049] A kind of embodiment of tilapia multi-parent hybridization seed production method of the present invention, described seed production method comprises the following steps:
[0050] (1) take the female fish in the new Jifu strain Nile tilapia as the female parent, and the male fish in the Sudan strain Nile tilapia as the male parent to carry out intraspecific hybridization to obtain the offspring generation Nile tilapia;
[0051] (2) With the female fish in the Nile tilapia of the daughter generation obtained in step (1) as the female parent, the male fish in the Maoming strain Oria tilapia is the male parent to carry out interspecific hybridization to obtain hybrid offspring Generation, namely said tilapia.
Embodiment 3
[0053] A kind of embodiment of tilapia multi-parent hybridization seed production method of the present invention, described seed production method comprises the following steps:
[0054] (1) take the female fish in the new Jifu strain Nile tilapia as the female parent, and the male fish in the Sudan strain Nile tilapia as the male parent to carry out intraspecific hybridization to obtain the offspring generation Nile tilapia;
[0055](2) With the female fish in the Nile tilapia of the first generation obtained in step (1) as the female parent, and the male fish in the Israeli strain Oria tilapia as the male parent, carry out interspecific hybridization to obtain hybrid offspring Generation, namely said tilapia.
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