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Haliotis discus hannnai and haliotis iris interspecific hybrid production method

A technology of wrinkled abalone and hybrid seed production, applied in application, climate change adaptation, fish farming, etc., can solve problems such as low fertilization rate and hatching rate, and achieve the effect of easy to master, low experience requirement, and simple operation.

Inactive Publication Date: 2016-04-20
山东大学(威海)
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AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problems of low fertilization rate and hatching rate in the existing hybridization technology, and provide a hybrid seed production method between black-footed abalone and wrinkled disc abalone with high fertilization rate and hatching rate

Method used

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Experimental program
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Effect test

Embodiment 1

[0026] Take wrinkled pan abalone with good individual vitality and no muscle damage as the female parent and black-footed abalone as the male parent. Among them, the average shell length of the wrinkled pan abalone is 9.5cm, and the average shell length of the black-footed abalone is 15.2cm; adjust the seawater Simultaneously promote the ripening of wrinkled abalone and black-legged abalone by temperature. The gonadal ripening temperature of wrinkled abalone is 20-22°C, and that of black-footed abalone is 15-17°C. During the ripening process, the seed abalone The male and female are placed in different parent abalone breeding ponds for breeding. The sex characteristics of the parent abalones in each parent abalone cultivation pond must be single, and the male and female cannot be mixed in the same parent abalone cultivation pond. The breeding density is 30 / m3. Feed the abalone with kelp; after the gonads of the abalone species mature, dry the gonad-developed wrinkled abalone in...

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Abstract

The invention relates to a haliotis discus hannnai and haliotis iris interspecific hybrid production method with a higher fertility rate and a higher hatching rate. According to the interspecific hybrid production method, Chinese haliotis discus hannnai is taken as female parent abalone, and Zelanian haliotis iris is taken as father parent abalone; the female parent abalone and the father parent abalone are put into different parent abalone cultivation ponds for cultivation; the female haliotis discus hannnai with the gonad fully developed is subjected to spawning induction with a method combining shady drying and ultraviolet irradiation seawater stimulation until the female haliotis discus hannnai lays eggs, and the male Zelanian haliotis iris with the gonad fully developed is subjected to spawning induction with a method combining shady drying and hydrogen peroxide seawater soaking until the male Zelanian haliotis iris releases sperms; fertilized eggs of haliotis discus hannnai and haliotis iris hybrids are obtained in the following steps: the eggs of the haliotis discus hannnai and the sperms of the haliotis iris are taken respectively and mixed, the temperature of seawater where fertilization is performed is adjusted, and the eggs are washed after the fertilized eggs are split to two cell stages; the haliotis discus hannnai and haliotis iris hybrid fries can be obtained with conventional hatching and later cultivation methods.

Description

technical field [0001] The invention relates to a method for hybrid seed production of shellfish, in particular to a method for hybrid seed production between species of abalone wrinkle and black-footed abalone. Background technique [0002] It has far-reaching theoretical significance and broad application prospects to use genetic improvement technology to cultivate fine varieties for shellfish farming. Hybrid breeding technology belongs to genetic improvement technology. Hybridization generally refers to the mating between two different types of parents above a variety or strain. The mating between two parents with a taxonomic status above a species is called interspecific hybridization. The hybrid individuals cultivated through cross breeding will have higher heterosis in terms of growth potential, survival rate and disease resistance, and can rapidly and significantly increase the yield and survival rate of the hybrids. [0003] Abalone is a kind of marine shellfish, kn...

Claims

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Application Information

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IPC IPC(8): A01K61/00A01K61/51
CPCY02A40/81
Inventor 侯旭光张海涛郭战胜梅俊学
Owner 山东大学(威海)
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