Haliotis sieboldii and Haliotis discus hannai interspecific hybridization seed production method

A wrinkled plate abalone and hybrid seed production technology, which is applied in the fields of medical science, climate change adaptation, animal delivery, etc., can solve the problems of low fertilization rate and hatching rate, achieve easy mastering, increase hybrid fertilization rate and hatching rate, experience undemanding effects

Active Publication Date: 2008-04-30
福建闽锐宝海洋生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problems of low fertilization rate and hatching rate existing in the existing hybridization technology, and to provide a method for interspecies hybridization of Siberian abalone and rugosa abalone with high fertilization rate and hatching rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] When the natural seawater temperature is 25°C, the western abalone and wrinkled abalone with good individual vitality and no muscle damage are taken as seed abalones. Abalones are placed in different parental abalone breeding pools according to male and female for breeding. The sex characteristics of parental abalones in each parental abalone breeding pool must be single, and mixed female and male cannot be stocked in the same parental abalone breeding pool. The cultivation density is 20 / m 3 , the composition of the bait fed to the parent abalone and its content according to the mass ratio are kelp 65, Jiangli 40, Ulva 25, laver 26 and spirulina 2; After 70 minutes, the ultraviolet-treated seawater with an irradiation intensity of 1000mWh / L was injected, and the seawater temperature was 22°C. The ultraviolet-treated seawater was replaced every 50 minutes until the male and female abalone spawned and ejaculated. During the process of artificial induction, the male and f...

Embodiment 2

[0031] When the natural seawater temperature is 26°C, take the western abalone and the wrinkled abalone with good individual vitality and no muscle damage as the seed abalone. The average shell length of the western abalone is 7.56cm, and the average shell length of the wrinkled abalone is 8.67cm: Abalones are placed in different parental abalone breeding pools according to male and female for breeding. The sex characteristics of parental abalones in each parental abalone breeding pool must be single, and mixed female and male cannot be stocked in the same parental abalone breeding pool. The cultivation density is 15 / m 3, the composition of the bait fed to the parent abalone and its content according to the mass ratio are 75% kelp, 45% Jiangli, 35% Ulva, 30% seaweed and 3 spirulina; 60min, then inject ultraviolet-treated seawater with an irradiation intensity of 900mWh / L, the seawater temperature is 20°C, and replace the ultraviolet-treated seawater every 60min until the male ...

Embodiment 3

[0033] When the natural seawater temperature is 20°C, take western abalone and wrinkled abalone with good individual vitality and no muscle damage as seed abalone. Abalones are placed in different parental abalone breeding pools according to male and female for breeding. The sex characteristics of parental abalones in each parental abalone breeding pool must be single, and mixed female and male cannot be stocked in the same parental abalone breeding pool. The cultivation density is 17 / m 3 , the composition of the bait fed to the parent abalone and its content according to the mass ratio are 70% kelp, 50% Jiangli, 25% Ulva, 30% seaweed, and 5% spirulina; After 70 minutes, ultraviolet-treated seawater with an irradiation intensity of 1000mWh / L was injected, and the seawater temperature was 21°C. The ultraviolet-treated seawater was replaced every 60 minutes until the male and female abalone spawned and ejaculated. During the process of artificial induction, the male and female ...

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Abstract

An interbreeding producing method of haliotis sieboldii and haliotis discus hannai relates to a cultivation method for seashells and provides a high-fertilization-ratio and high-hatching-ratio interbreeding producing method of haliotis sieboldii and haliotis discus hannai. haliotis sieboldii are taken as parent ormers and haliotis discus hannai are taken as parent ormers when the temperature of natural seawater is 22-26 DEG C, ormers are respectively arranged in different cultivation pools of parent ormers according to sex for cultivation, individuals with mature gonad are expedited to produce with shade drying combined with the stimulation method of using ultraviolet to irradiate seawater until female and male parent ormers lay ova and sperm. The sperm of haliotis sieboldii and the ova of haliotis discus hannai are taken and combined, the pH of seawater for fertilized ova is adjusted, and then ova are washed when fertilized ova divide to the binary division period. Fertilized ova produced by interbreeding female haliotis sieboldii and male haliotis discus hannai can be achieved by that the sperm of haliotis discus hannai and the ova of haliotis sieboldii are taken and combined, the pH is adjusted, and then ova are washed when fertilized ova divide to the binary division period. Finally, ova are cultivated according to normal batching and carry-on cultivation methods.

Description

technical field [0001] The invention relates to a method for cultivating shellfish, in particular to a method for interspecies hybridization of Siberian abalone and wrinkled pan abalone. Background technique [0002] It has far-reaching theoretical significance and broad application prospects to use genetic improvement technology to cultivate fine varieties for shellfish farming. Hybrid breeding technology belongs to genetic improvement technology. Hybridization generally refers to the mating between two different types of parents above a variety or strain. The mating between two parents with a taxonomic status above a species is called interspecific hybridization. The hybrid individuals cultivated through cross breeding will have higher heterosis in terms of growth potential, survival rate and disease resistance, and can rapidly and significantly increase the yield and survival rate of the hybrids. [0003] Abalone is a kind of marine shellfish, known as the first of the e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00A61D19/00A01K61/51
CPCY02A40/81
Inventor 骆轩柯才焕游伟伟
Owner 福建闽锐宝海洋生物科技有限公司
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