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Identifying method for pleurotus nebrodensis-pleurotus eryngii interspecific hybrid

An identification method, the technology of Pleurotus eryngii, applied in the field of bioengineering, can solve the problems of flavor, growth cycle biotransformation rate and production management conditions, tissue compactness, flower shape and color, etc., to increase new variety resources, DNA marker strips The effect of less bands and high accuracy

Inactive Publication Date: 2012-08-01
SHANGHAI BAIRONG VELVET MUSHROOM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the tissue density, taste, and flower shape and color of Pleurotus eryngii are better than those of Pleurotus eryngii, while the flavor, growth cycle, biotransformation rate and production management conditions are inferior to Pleurotus eryngii

Method used

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  • Identifying method for pleurotus nebrodensis-pleurotus eryngii interspecific hybrid
  • Identifying method for pleurotus nebrodensis-pleurotus eryngii interspecific hybrid

Examples

Experimental program
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Embodiment 1

[0041] (1) Selection of parental strains of Bailing mushroom and Pleurotus eryngii, preparation of protoplasts, fusion of protoplasts:

[0042] Select high-quality, high-yield Bailing mushroom strains and Pleurotus eryngii strains as hybrid parents;

[0043] 1. Preparation of protoplasts of the monospore strain of Bailing mushroom: activate the monospore strain of Bailing mushroom with PDA slant medium, transfer it to a 250 mL wide-mouthed triangular flask containing 100 mL of PDA liquid medium, and culture it statically at 25 ° C for 6 ~ 7d, use a pipette gun to take 5-7 mL of culture solution into a 250-mL wide-mouth Erlenmeyer flask containing 100 mL of PDA liquid medium, and culture it at 25°C for 6-7 days, pick 0.3-0.6 g of young mycelium and put it into In a 5mL centrifuge tube, centrifuge at 5000r / min for 10min, discard the centrifuged liquid, add 300ul lysozyme enzyme solution into the 5mL centrifuge tube, put it in a water bath at 28-30°C for 2-2.5h, and use a syringe...

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Abstract

The invention belongs to the field of biological engineering, and is used for solving the technical problem of difficulty in screening a pleurotus nebrodensis-pleurotus eryngii interspecific hybrid in the prior art. The invention provides an identifying method of the pleurotus nebrodensis-pleurotus eryngii interspecific hybrid. The method comprises the following steps of: preparing a strain protoplast; culturing the protoplast; culturing and collecting hyphae in the protoplast; extracting genome DNAs (Deoxyribonucleic Acids) in the hyphae; performing ITS-PCR (Internal Transcribed Spacer-Polymerase Chain Reaction) amplification on the extracted DNAs; performing specific enzyme splicing on an amplification product; and performing electrophoresis on an enzyme splicing product. If DNA marking strips of which the molecular weights are 675-685bp, 585-595bp and 85-95bp are generated according to electrophoresis detection, the strain is an interspecific hybrid of pleurotus nebrodensis and pleurotus eryngii. The identifying method disclosed by the invention has the advantages of short detection time, high accuracy, easiness for judging and high stability.

Description

technical field [0001] The invention belongs to the field of bioengineering, and relates to an identification method for edible fungus hybrids, in particular to an identification method for interspecific hybrids of Bailing mushroom and Pleurotus eryngii. Background technique [0002] Bailing Mushroom ( Pleurotus nebrodensis (Inzengae) Quél) Saprophytic or parasitic on the rhizome base or root of the perennial herb Ferula in the family Umbelliferae. Pleurotus eryngii ( Pleurotus eryngii (DC.:Fr.) Quél) occurs in the genus Eryngium of Umbelliferae, on dead plants of Eryngium. Both belong to the genus Pleurotus. [0003] Bailing mushroom and Pleurotus eryngii not only have high nutritional value, good taste and flavor, but also have high medicinal value, and the market prospect is broad. However, the tissue density, taste, and flower shape and color of Bailing mushroom are better than that of Pleurotus eryngii, while the flavor, growth cycle, biotransformation rate and p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 杨开耀邓优锦余作茂
Owner SHANGHAI BAIRONG VELVET MUSHROOM
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