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Novel isolated culture method for cherry intraspecies and intraspecific hybrid embryo

A technology of in vitro culture and hybrid embryos, applied in the field of agricultural biology, can solve the problems of inability to form seedlings, germination rate, incomplete embryo development of early-maturing varieties, etc., and achieve the effects of considerable economic benefits, short production cycles and good market prospects.

Inactive Publication Date: 2013-09-11
SICHUAN AGRI UNIV
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The object of the present invention is to provide a novel in vitro culture method for hybrid embryos of hybrids within and between species of cherry, aiming to solve the problem of abortion or degeneration of hybrid embryos of hybrid hybrids in the middle of cherry species, and the incomplete development of early-maturing varieties of hybrid embryos leading to failure of conventional sowing. Seedlings or very low germination rate are two major obstacles, which provide an important guarantee for the selection of new cherry varieties with self-compatibility, large fruit, high quality, early maturity, storage and transportation, and strong resistance

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  • Novel isolated culture method for cherry intraspecies and intraspecific hybrid embryo

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Embodiment 1

[0023] A new type of in vitro culture method for cherry intraspecific and interspecific hybrid embryos, as shown in the attached figure 1 As shown, it includes the following 8 steps: S101, hybrid acquisition; S102, hybrid pretreatment; S103, establishment of hybrid sterile materials; S104, hybrid embryo subculture; S105, hybrid embryo seedling cultivation; S106, hybrid embryo Rooting cultivation; S107, hardening and transplanting; S108, transplanting in the field.

[0024] S101. Obtaining hybrids: picking Sichuan wild Chinese cherry Beichuan Guixi Community No. 3 (small tree, red flowers) as the male parent of bell flowering period (petals have been loosened but not opened), brought back indoors to pick off anthers, and placed in The pollen was dry-popped at 20°C, and the pollen split from the anthers was collected in clean vials, labeled and stored in a dry environment for later use, and the viability of the pollen was tested before pollination. In the blooming stage of the ...

Embodiment 2

[0035] A new type of in vitro culture method for cherry intraspecific and interspecific hybrid embryos, as shown in the attached figure 1 As shown, it includes the following 8 steps: S101, hybrid acquisition; S102, hybrid pretreatment; S103, establishment of hybrid sterile materials; S104, hybrid embryo subculture; S105, hybrid embryo seedling cultivation; S106, hybrid embryo Rooting cultivation; S107, hardening and transplanting; S108, transplanting in the field.

[0036] S101. Obtaining hybrids: Picking Sichuan wild Chinese cherry Beichuan Guixi Juqun No. 2 (larger tree, red flowers) in the flowering stage of the bell as the male parent, removing the petals and taking the anthers indoors, and drying at 20°C Popping pollen, the pollen split from the anthers is collected in clean vials, labeled and stored in a dry environment for later use, and the pollen viability is tested before pollination. Also in the blooming stage of the female parent, the Chinese cherry local cultivar...

Embodiment 3

[0047] A new type of in vitro culture method for cherry intraspecific and interspecific hybrid embryos, as shown in the attached figure 1 As shown, it includes the following 8 steps: S101, hybrid acquisition; S102, hybrid pretreatment; S103, establishment of hybrid sterile materials; S104, hybrid embryo subculture; S105, hybrid embryo seedling cultivation; S106, hybrid embryo Rooting cultivation; S107, hardening and transplanting; S108, transplanting in the field.

[0048] S101. Obtaining hybrids: Picking Sichuan wild Chinese cherry Beichuan Guixi Juqun No. 5 (small tree, red flowers) bell flowering period as the male parent, removing petals and anthers indoors, drying and popping at 20°C The pollen split from the anthers was collected in clean vials, labeled and stored in a dry environment for later use, and the viability of the pollen was tested before pollination. Similarly, in the blooming stage of the female parent, the European sweet cherry cultivar 'Hongdeng' (kidney-s...

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Abstract

The present invention discloses a novel isolated culture method for cherry intraspecies and intraspecific hybrid embryo. The novel isolated culture method comprises: hybrid obtaining, a hybrid pretreatment, hybrid sterile material establishment, hybrid embryo subculture, hybrid embryo strong seedling culture, hybrid embryo rooting culture, seedling exercising transplantation, and transplanting into field. According to the present invention, the hybrid embryo isolated culture method is utilized, such that a male parent for new cherry variety breeding and having excellent characteristics and a female parent for new cherry variety breeding and having excellent characteristics can be effectively screened, it is ensured that the bred filial generation has the same excellent characteristics of the male parent and the female parent, and the bred hybridization variety has targeting property and universality; and a large number of cherry seedlings can be rapidly bred in a low cost manner, and a production period is short, such that economic benefits are good, and good market prospects are provided.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to a method for in vitro culture of novel cherry intraspecific and interspecific hybrid embryos. Background technique [0002] Cherries belong to the deciduous trees of the genus Prunus in the Rosaceae family. Classification according to fruit structure Cherry fruit belongs to drupe fruit. Due to the short development period and early maturity period of cherry fruit, the fruit is listed in the off-season of the fresh fruit market. The fruit is beautiful in color, rich in nutrition, and suitable for fresh food and processing. It is known as a treasure among fruits. There are more than one hundred species of Prunus genus, and the main economically cultivated fruit trees are Chinese cherry, European sweet cherry and European sour cherry. Chinese cherry is native to my country and has many varieties. It has a long history of cultivation and a certain cultivation a...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 邓群仙袁航王小蓉王永清陈涛罗娅杜寒梅
Owner SICHUAN AGRI UNIV
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