Method for producing tetraploid oysters and interspecific hybridization triploid oysters

A technology of triploid and tetraploid, applied in the field of genetic breeding of aquaculture species, can solve problems such as low sperm motility of tetraploid oysters, difficulty in interspecific fertilization, and no large-scale production of triploid oysters, etc., to achieve excellent Effects of Traits and Commercial Value, Large Maturity

Active Publication Date: 2021-11-23
青岛前沿海洋种业有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Different scholars have conducted preliminary research on interspecific hybridization of diploid oysters, but due to the low sperm motility of tetraploid oysters and the difficulty of interspecific fertilization, there is no effective method for large-scale production of interspecific hybrid triploid oysters with excellent traits.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Ripening of different triploid oysters:

[0030] Take 100 identified 2-year-old artificially induced triploid Pacific oysters, triploid Fujian oysters and triploid Hong Kong oysters, respectively, and place them in a broodstock culture tank with seawater at 16°C for 1 day. Pacific oysters and Fujian oysters use 32 parts per thousand of sea water, and Hong Kong oysters use 16 parts per thousand of sea water. From the second day, the temperature was increased by 5°C every day, and reached 26°C on the third day, and kept at 26°C for 5 days, and the bait was cast 8 times a day according to the existing conventional method, and 100% of the water was changed by pouring the pond. From the 8th day, the water temperature was lowered to 23°C, and then the bait was fed 8 times a day according to the existing conventional method, and the water was changed by 100%; all reached sexual maturity on the 38th day, and then obtained mature Pacific triploids respectively. Oysters, Fujian ...

Embodiment 2

[0032] Open 96 triploid Pacific oysters and obtain 46 female oysters. After dismantling and taking the eggs and washing them, put them into 46 beakers respectively. Take samples from each beaker and measure the diameter of 10 eggs to get the average value. The eggs in 15 beakers were mixed to obtain a total of 26 million eggs, which were diluted to 10,000 eggs / ml. Open 48 diploid Pacific oysters, obtain 28 male shellfish, dissect the largest 15 to get sperm, filter and mix in equal amounts. From 5 minutes after fertilization, treat the fertilized eggs with CB (0.5mg / L) to inhibit the first polar body, rinse after 15 minutes, and then put them into 520 liters of water to hatch. After hatching, press 10 D-type cloth pools per ml, normal culture. All treatment operations were performed at 25°C with sand-filtered and UV-sterilized seawater with a salinity of 32 ppm.

[0033] At the same time, the remaining eggs with average diameter were mixed, and used as the control of the lar...

Embodiment 3

[0038] Open 94 triploid Fujian oysters to obtain 51 female oysters, dissect the eggs, wash them and put them into 51 beakers respectively, measure the diameter of 10 eggs from each beaker and take the average value, and take the egg with the largest diameter The eggs in the first 15 beakers were mixed to obtain a total of 17 million eggs, which were diluted to 10,000 eggs / ml. 45 diploid Fujian oysters were opened to obtain 22 male shellfish, and the largest 15 male shells were dissected to obtain sperm, which were filtered and mixed in equal amounts. From 5 minutes after fertilization, treat fertilized eggs with CB (0.5mg / L) or 6DMAP (50mg / L) to inhibit the first polar body, rinse after 15 minutes, and then put them into 350 liters of water for incubation. 10 D-type cloth pools were cultured normally. All treatment operations were performed at 25°C with sand-filtered and UV-sterilized seawater with a salinity of 32 ppm.

[0039] After the above-mentioned normal culture, the ...

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PUM

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Abstract

The invention belongs to the technical field of genetic breeding of aquaculture varieties, and relates to a method for producing tetraploid oysters and a method for interspecific hybridization of triploid oysters by using the tetraploid oysters. The triploid oysters are stimulated for 5-8 days at the high temperature of 26-28 DEG C, ripening is promoted for 30-40 days at the water temperature of 22-24 DEG C after stimulation, and ova of female oysters with the largest diameter are selected after ripening; the selected ova and sperms of diploid oysters are fertilized for 5-8 minutes, then an inhibitor is added to inhibit first polar body discharge of the fertilized ova, and then a large number of tetraploid oysters are stably obtained for optimization. After strong optimization, high-concentration sperms are used for carrying out hybrid fertilization on excellent tetraploid oysters and excellent diploid oysters in different species, and interspecific hybrid triploid oysters with excellent breeding traits are obtained. According to the method for producing the tetraploid oysters and the method for interspecific hybridization of the triploid oysters by using the tetraploid oysters, the method for producing the tetraploid oysters and the method for interspecific hybridization of the triploid oysters by using the tetraploid oysters can be used for stably and efficiently producing the tetraploid oysters and the hybrid triploid oysters with the excellent breeding traits, can be widely applied to genetic breeding of oysters and shellfishes, and improves the shellfish breeding industry.

Description

technical field [0001] The invention belongs to the technical field of genetic breeding of aquaculture varieties, and relates to a method for producing tetraploid oysters and a method for interspecies hybridization of triploid oysters using tetraploid oysters. Background technique [0002] Triploid shellfish has three sets of chromosomes, so it has fast growth and excellent breeding shape of sterility, and has high application value in shellfish farming. The commercial use of triploid molluscs depends on the availability of tetraploid molluscs. Tetraploid molluscs have four sets of chromosomes and can be crossed with diploids to produce 100% triploid molluscs on a large scale. But how to obtain viable tetraploid shellfish is a challenge. [0003] Tetraploid oysters can be obtained by fertilizing triploid oyster eggs with normal diploid sperm and inhibiting polar body release (CN1144454A, CN100415085C, CN107494358A, CN109730008A). Tetraploid oysters can also be obtained by...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/54
CPCA01K61/54Y02A40/81
Inventor 李雅林李晓喻付杰李胜洪郭希瑞
Owner 青岛前沿海洋种业有限公司
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