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Method for artificially developing novel brassica napus

A new type of Brassica napus technology, applied in botany equipment and methods, plant genetic improvement, angiosperms/flowering plants, etc., can solve the problem of narrow genetic basis of Brassica napus, unfavorable utilization of heterosis among genomes, and difficult selection of plants traits and other issues

Inactive Publication Date: 2009-08-26
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

Artificially synthesizing Brassica napus through this method has defects such as difficult hybridization compatibility, slow progress in genetic stability of hybrid offspring (synthetic Brassica napus), and small genetic variation. Both parents are vegetable crops, so it is difficult to select excellent plant traits
In addition, there are currently two methods of breeding new types of Brassica napus in breeding, that is, through interspecific hybridization of Brassica napus and Brassica napus, Brassica napus and Brassica napus, respectively, to obtain Brassica napus in the offspring; The hybridization compatibility of these two ways is good, and the work is simple, but one of the parents of the two ways is a natural Brassica napus, so the genetic basis of Brassica napus obtained from a large number of segregating offspring is relatively narrow, although domestic In addition, a large number of new materials and new varieties used in production have been obtained in this way, but compared with natural Brassica napus, the genetic distance of these materials is relatively close, which is not conducive to the utilization of heterosis between subgenomes
[0004] So far, there has been no report on the new artificial synthesis method for obtaining new types of Brassica napus by concentrating the excellent traits of Brassica oleracea and Brassica napus

Method used

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  • Method for artificially developing novel brassica napus

Examples

Experimental program
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Effect test

Embodiment 1

[0018] When rapeseed is in full flowering stage in February-April, select the vigorous mustard type rape as the female parent, remove the flower buds that have bloomed and are too small, and emasculate the flower buds that will bloom in the past 1-2 days, and use a mass percentage concentration of 10%. Apply NaCl to the stigma, wait for the salt water to dry naturally, and then infuse pollen taken from healthy kale plants. After pollination, quickly cover the stigma with a paper bag to prevent the introduction of foreign pollen. Repeat pollination the next day.

[0019] About 20 days after pollination, take immature young ovules, disinfect the surface with 75% alcohol under aseptic conditions, and then disinfect with sodium hypochlorite with a mass percentage concentration of 10%-15% for 15-20 minutes, and then inoculate into MS On the medium, after it germinates, it is transferred to the rapid propagation medium for rapid propagation to obtain clones. Around October-November...

Embodiment 2

[0023] Embodiment 2: Cabbage is selected from head cabbage, and the above-mentioned example operations are carried out at the same time.

Embodiment 3

[0024] Embodiment 3: Brassica kale is selected as the cabbage, and the above-mentioned example operations are carried out at the same time.

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Abstract

The present invention provides a method for artificially developing novel brassica napus. The excellent characters of cabbage and mustard type rape are integrated for obtaining novel brassica napus. The hybridization compatibility when the mustard type rape and cabbage are used for executing interspecific hybridization is higher than the hybridization compatibility when the Chinese cabbage and cabbage are used for executing interspecific hybridization. The interspecific hybrid is easily obtained, and simultaneously, the advantages of drought resistance, cold resistance, excellent strain type, etc. of the mustard type rape can be introduced into the brassica napus. The method of the invention also has the advantages of stable heredity, high speed, large area of character variation and facilitation for selecting the brassica napus with excellent charcter. Furthermore, the chromosomes of AC chromosome set of brassica napus obtained from the method of the invention respectively come from the mustard type rape and cabbage, have larger difference from the prior brassica napus. The use of hybrid vigor of genome of brassica napus is facilitated and the production level of cabbage is increased.

Description

technical field [0001] The present invention relates to the technical field of crop breeding, in particular to a new method of artificial synthesis of Brassica napus (2n=4x=AACC=38), specifically using Brassica napus (2n=2x=C°C°=18) and Brassica napus Rape (2n=4x=A J A J B J B J =34) A method for artificially synthesizing novel Brassica napus (2n=4x=A'A'C'C'=38) by interspecific hybridization. Background technique [0002] As one of the main oil crops in the world, rapeseed is not only an important edible oil source and protein feed, but also an important industrial raw material and nectar source plant. my country is one of the first countries to cultivate rapeseed, but in the past it was mainly the cultivation of cabbage-type rapeseed. In the 1930s, Brassica napus began to be introduced and cultivated from North Korea, Japan and the United Kingdom. Now it is widely distributed all over the country, especially in the main producing areas of rapeseed in the provinces of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04A01H5/00
CPCY02A40/10Y02A40/132
Inventor 周清元李加纳殷家明
Owner SOUTHWEST UNIVERSITY
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