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Method using young embryo to save and obtain interspecies hybrid of hydrangea macrophylla

A technology of young embryo rescue and hydrangea, applied in the field of biotechnology breeding, can solve the problems of easy abortion, inability to obtain hybrid offspring, etc., and achieve the effect of avoiding premature aging

Inactive Publication Date: 2016-04-20
杨玉勇 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The cultivation of new hydrangea varieties is mostly achieved through intraspecific hybridization, and interspecific hybridization is a distant hybridization. In the natural state, it is impossible to obtain hybrid offspring due to easy abortion.

Method used

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  • Method using young embryo to save and obtain interspecies hybrid of hydrangea macrophylla
  • Method using young embryo to save and obtain interspecies hybrid of hydrangea macrophylla
  • Method using young embryo to save and obtain interspecies hybrid of hydrangea macrophylla

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Effect test

Embodiment 1

[0025] A. Disinfection of ovary: Cut off the incompletely developed ovary 60 days after pollination, store it at 4°C for 48 hours, take it out, wipe its surface twice with 75% alcohol, and rinse it with running water for 4 hours , to the ultra-clean workbench. Soak in 75% alcohol for 30 seconds, soak in 0.05% mercuric solution for 15 minutes, wash 6 times with sterile water, select the enlarged ovary, and cut off the base of the ovary with a sterile blade;

[0026] B. Inoculation: Under sterile conditions, inoculate the ovary that has been sterilized and cut off the base into 1 / 2MS+6-BA1.0mg / L+NAA1.0mg / L+sucrose 30g / L+agar 6.5g / L. Adjust the pH before bacteria to 5.8-6.0. The culture temperature is 22°C, the light intensity is 2000Lx, the light time is 12h / d, the air humidity is 70%, and the germination rate reaches 30-40% after 30 days of culture;

[0027] C. Proliferation culture: Inoculate the germinated immature embryos into 1 / 2MS+6-BA1.0mg / L+NAA1.0mg / L+sucrose 30g / L+aga...

Embodiment 2

[0037] A. Disinfection of ovary: cut off the incompletely developed ovary 70 days after pollination, store it at 8°C for 72 hours, take it out, wipe the surface with 75% alcohol for 3 times, and rinse with running water for 6 hours , to the ultra-clean workbench. Soak in 75% alcohol for 30 seconds, soak in 0.1% mercuric solution for 8 minutes, wash 6 times with sterile water, select the enlarged ovary, and cut off the base of the ovary with a sterile blade;

[0038] B. Inoculation: Under sterile conditions, inoculate the ovary that has been sterilized and cut off the base into 1 / 2MS+6-BA1.0mg / L+NAA0.5mg / L+sucrose 30g / L+agar 6.5g / L. Adjust the pH before bacteria to 5.8-6.0. The culture temperature is 25°C, the light intensity is 2000-2500Lx, the light time is 14h / d, the air humidity is 85%, and the germination rate reaches 30-40% after 45 days of culture;

[0039] C. Proliferation culture: inoculate the germinated immature embryos into 1 / 2MS+6-BA1.0mg / L+NAA0.5mg / L+sucrose 30g...

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Abstract

The invention provides a method using young embryo to save and obtain distant hybrid of hydrangea macrophylla. According to the provided method, non-hardy hydrangea macrophylla (diamond) is taken as the female parent and hardy H. arborescence Annabelle is taken as the male parent to carry out interspecies hybridization; ovary culture is performed to rescue young embryo; and the obtained hybrid offspring is subjected to morphological identification and SSR identification. The provided method can prevent the phenomenon of embryo premature senility, which is caused by incompatibility, of distant hybrid. A large amount of interspecies hybrid offspring can be obtained through tissue culture. The interspecies distant hybridization of hydrangea macrophylla is achieved; novel species, which have a strong performance on resisting cold and a high ornamental value, are created; and the breeding period of hydrangea macrophylla is largely reduced.

Description

technical field [0001] The invention relates to a method for obtaining hydrangea distant hybrids through in vitro culture of fertilized immature embryos, and belongs to the field of biotechnology breeding. Background technique [0002] Hydrangea ( Hydrangea macrophylla ), also known as hydrangea and hydrangea, is a plant of the genus Hydrangea in the family Saxifragaceae. Hydrangea ( Hydrangea ) There are about 85 species of plants in the world. There are 47 species and 11 varieties of hydrangeas in my country. Except for Hainan in the south, Heilongjiang and Jilin in the northeast, and Xinjiang in the northwest, they are distributed all over the country, especially from the southwest to the southeast. Most types of department. Hydrangea is native to China and Japan, and likes warm, humid and semi-shady environments, and is not very cold-tolerant. Avoid direct exposure to the hot sun in summer, otherwise the leaves will be easily scorched and yellow, and it is not suitab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
Inventor 杨玉勇
Owner 杨玉勇
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