Method and culture medium for obtaining distant hybrids of strawberry by in vitro rescue culture

Abstract

The invention discloses a method and culture medium for obtaining a strawberry distant hybrid through in-vitro rescue culture. According to the technology, cultivated strawberry varieties including JiuXiang, Sweet Charlie and FengXiang serving as a female parent or a male parent and wild Fragaria nilgerrensis serving as a male parent or a female parent are hybridized, and direct hybridization and reversed hybridization are simultaneously performed. By performing in-vitro culture on immaturate embryos obtained after hybrid pollination, taking out the embryos before embryo abortion and performing rescue culture on the embryos, decaying of distant hybrid embryos is avoided, a large number of distant hybrid embryos continue to develop into seedlings, seed selection is performed, and then the seed breeding period is shortened. Meanwhile, a large number of distant hybridization progenies are obtained, distant hybridization between Fragaria varieties is achieved, a batch of new germplasms are created, the culture period of the strawberry hybridization progenies is effectively shortened, and the adult seedling rate of the hybrid embryos can reach 91.67%.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method and a culture medium for obtaining distant hybrids of strawberry through rescue culture in vitro. Background technique [0002] Strawberry belongs to the Rosaceae (Rosaceae) genus Fragaria (Fragaria) perennial herbaceous fruit tree, ranking first in the world's small berry production, most countries have cultivation. Germplasm resources are the material basis of breeding. The great achievements of modern crop breeding mainly benefit from the discovery and utilization of a number of important germplasms, as well as the innovation and application of breeding technology. The collection, excavation and utilization of germplasm resources have been paid more and more attention by governments and breeders of various countries. On the one hand, due to people's attention to germplasm resources, more and more favorable genes and traits in plants have been discovered, and ...

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Problems solved by technology

People such as Wang D reported the research on the cultivation of immature embryos of strawberry, but they mainly studied the factors affecting the cultivation of immature embryos, and did not study the specific application in breeding technology (HortScience, 1984, 19: 71-72)

Due to the different sources and genetic backgrounds of parents selected in the process of distant hybrid breeding, there will be differences in the conditions of embryo induction, embryo germination, proliferation, rooting, and seedling formation in the process of in vitro culture of immature embryos. The successful application of in vitro culture of immature embryos in distant hybrid breeding has not been reported

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