Construction method and application of Mozi hybrid scallop maintainer line

A method for constructing a maintainer line, which is applied in the field of constructing a maintainer line of Mozi hybrid scallops, can solve the problems of inability to establish male sterile lines, maintainer lines and restorer lines, and achieves the effect of overcoming obstacles to self-fertilization and low cost.

Active Publication Date: 2015-08-05
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, in animal breeding, there have been no reports of male sterile and female fertile individuals at home and abroad so far, so it is impossible to establish male sterile lines, maintainer lines and restorer lines similar to crops for hybrid breeding

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1) Screening of male sterile individuals of Mozi hybrid scallops and breeding of their male parents: in the first year, 50 gonad-developed Gulf scallops and 50 purple scallops were selected, and each scallop was placed in the pre- Add seawater at 23°C to a separate container to spawn and ejaculate, collect the eggs of each Gulf scallop and the sperm and eggs of each purple scallop separately, add a small amount of Stir the semen of a single purple scallop to mix the sperm and eggs evenly, observe under a microscope so that there are 5-6 sperm around each egg, and place the fertilized eggs in a dark environment to hatch, that is, to obtain a generation of black purple hybrids. Establish at least 20 such hybrid families with the above method, and simultaneously fertilize the fertilized purple scallop sperm eggs to establish a corresponding number of purple scallop self-bred families, and carry out larvae cultivation, seedling preservation and cultivation according to the c...

Embodiment 2

[0025] In 2011, using the imported purple scallop and the Gulf of Mexico scallop from Zhanjiang, Guangdong, a first-generation orthogonal and a reciprocal family of interspecific hybrids were co-bred. According to actual measurements, the average shell height of the first generation of Mozi hybrid scallops (Gulf of Mexico scallop eggs fertilized with purple scallop sperm) is 52.22 mm, body weight is 25.49 grams, and column weight is 2.19 grams, while the average shell height of Gulf of Mexico scallops cultured in the same period is 42.02 mm, with a body weight of 14.80 grams and a column weight of 1.13 grams. Compared with the Gulf of Mexico scallops of the same period, the first generation of Mozi hybrids had a shell height of 24.3%, a body weight of about 72.2%, and a column weight of 93.8%. In the spring of 2012, after promoting the maturation of the above-mentioned first-generation scallops, select scallops that are large and only develop in the female area. After drying in...

Embodiment 3

[0028] In 2014, a total of 16 first-generation hybrid families of Gulf scallop eggs and purple scallop sperm were established using Gulf scallops and purple scallops, and self-bred families of purple scallops for sperm were established. According to the method in Example 1, you can The male sterile line of Mozi hybrid scallop and its corresponding maintainer and restorer lines are selected within 1-3 years to realize the commercial production of Mozi hybrid scallop seedlings.

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Abstract

The invention relates to a construction method and application of a argopecten irradians concentricus and argopecten purpuratus hybrid scallop maintenance line. The maintenance line can enable corresponding male-sterile line offsprings of argopecten irradians concentricus and argopecten purpuratus hybrid scallops to still have argopecten purpuratus lines with the characteristics of male sterility and egg fertility, wherein the argopecten irradians concentricus and argopecten purpuratus hybrid scallops are the hybrid offsprings of argopecten irradians concentricus eggs and argopecten purpuratus sperms. The construction method comprises the following steps of firstly, constructing lots of argopecten irradians concentricus and argopecten purpuratus hybrid scallop generation lines, and finding male-sterile individuals for select breeding a male-sterile line; secondly, using sperms of the male parent argopecten purpuratus scallops to continuous back cross, and respectively select breeding the offsprings to be male-sterile line-maintenance line combination of the male-sterile individuals; finally, hybridizing the male-sterile line and the maintenance line to produce male-sterile line scallops, and using the maintenance line to produce argopecten irradians concentricus and argopecten purpuratus hybrid scallop commercial fingerlings. According to the construction method and the application of the argopecten irradians concentricus and argopecten purpuratus scallop maintenance line, the maintenance line of the scallops is constructed in animals for the first time, and the obstacle of autofertilization in hermaphrodite scallop hybridization is effectively overcome.

Description

technical field [0001] The invention belongs to shellfish breeding technology-a method for cultivating commercial hybrid scallop seedlings, and in particular relates to a method for constructing and application of a maintainer line of Mozi hybrid scallop (the hybrid offspring of Gulf of Mexico scallop ova and purple scallop sperm). Background technique [0002] The Gulf of Mexico scallop (Argopecten irradians concentricus) is native to the coast of the Gulf of Mexico in the United States and was introduced to my country in the 1990s. The Gulf of Mexico scallop grows fast and adapts to a wide range of temperatures. It is especially suitable for high-temperature sea areas and has strong stress resistance. It is very suitable for farming in the southern seas of my country. It is currently the main scallop cultured in the southern seas. However, due to the long-term inbreeding after the introduction, the germplasm of the Gulf of Mexico scallop has been seriously degraded during ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 王春德马斌孙奎府赵玉明刘博周顺王文琪赵侠苗国英
Owner QINGDAO AGRI UNIV
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