Cultivation method of transgenic rice sterile line based on CYP704B2 gene

Abstract

The invention discloses a cultivation method of a transgenic rice sterile line based on a CYP704B2 gene. The cultivation method includes the following steps: A, obtainment of an EYFP gene expression cassette; B, amplification of a wheat pollen lethal gene Ki; C, amplification of a wheat pollen specific promoter Pg47; D, obtainment of a rice gene expression cassette CYP704B2; and E, linkage of allgene expression elements. The step E which is the linage of all the gene expression elements includes the following steps: firstly, linking the EYFP gene expression element, the wheat pollen lethal gene Ki and the wheat pollen specific promoter Pg47 to a pCAMBIA1300 vector, and then introducing and linking a complete rice CYP704B2 gene to the composite vector. An F1 generation heterozygote followsthe Mendelian separation law in a self-fertilization process, and produced progenies contain both the heterozygosis capable of maintaining three linkage genes and the sterile line without fertility.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering genetics and breeding, in particular to a method for cultivating a transgenic rice sterile line based on CYP704B2 gene. Background technique [0002] Rice is native to tropical Asia, and after being widely planted in China, it gradually spread to all parts of the world. Rice is the staple food of nearly half of the world's population. In addition to being edible, rice can also be used for wine making, sugar refining and as an industrial raw material. Years of production practice have shown that hybrid rice can generally increase yield by more than 20% compared with conventional rice, so hybrid rice shows great potential for increasing yield. [0003] The development of hybrid rice depends on the cultivation of male sterile lines of hybrid rice. Research on hybrid rice in my country began in the 1960s, and it began to be planted on a large scale in the 1970s. The first-generation hyb...

AI Technical Summary

Problems solved by technology

In the three-line method, the available parents with good shape are limited, the combination is not free, and the frequency of wild-lost maintenance lines is low

However, the rice CYP704B2 gene mutation will cause the tapetum cells to fail to degrade in time, and the programmed death will be delayed, so the callose enzyme secreted by the tapetum will affect the development and separation of microspores, and finally show sterility

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