Method for culturing pure laver line

A cultivation method and technology of seaweed, applied in botany equipment and methods, cultivation, plant cultivation, etc., can solve the problems that chimeric cells cannot be ruled out, strains are not pure, etc.

Inactive Publication Date: 2002-05-01
刘必谦 +1
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  • Summary
  • Abstract
  • Description
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Problems solved by technology

However, this method cannot rule out the existence of chimeric cells in a small area, so it still faces the problem of strain impurity

Method used

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Examples

Experimental program
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Effect test

Embodiment Construction

[0012] Embodiments of the present invention are further described in detail below.

[0013] 1. Select fresh immature laver leaves; or partially mature laver leaves on the edge, cut off the edge as much as possible, and keep the immature area in the center. Remove miscellaneous algae, treat with 0.7% KI and 1% penicillin for 10 minutes respectively, and then rinse with sterile sea water several times for aseptic operation. Cut the center area of ​​the treated blade to 1mm 2 Little pieces left and right.

[0014] 2. Use protein extracts from herbivorous shellfish in the intertidal zone of the ocean, such as snails, snails, oysters, etc., with a concentration of 1%, and add NaCl or glucose to make the final concentration of NaCl 1.5mol / L or glucose 2mol / L, will process 1mm in 1 step 2 The small piece is enzymolyzed at 20°C, pay attention to observe the enzymatic hydrolysis, when the 5-6 layers of cells around the leaves are loose, they can be rinsed with sterile sea water for...

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Abstract

A method for breeding high-purity laver line includes decomposing the immature laver leaf by mussel protein extract liquor to generate its unicells or protoplasts, culturing or inducing chromosomal multiplication to obtain pure line of diploid filamnets, culturing until sexual mature, self fertilization to generate homozygous diploid filaments, and culturing the certified seeds or seedlings. Its advantages are excellent individual characteristics, and high yield and quality of laver.

Description

technical field [0001] The invention relates to the technical field of cultivation and production of laver cells. Background technique [0002] Due to its rich nutrition and great potential medicinal value, laver has become one of the two major marine algae industries in my country, and its output ranks third in the world. In 2000, the total output value of laver in my country reached about 200 million yuan. In my country, the history of people eating seaweed can be traced back to more than a thousand years ago, and the history of seaweed breeding has been hundreds of years. In recent decades, with the development of laver cultivation industry, people have higher and higher requirements for the quality of laver varieties. But so far, there is no report of cultivating a pure line of laver that has excellent characters and can be stably inherited. This current situation is not commensurate with its industrial scale, and is also extremely unfavorable to the development of la...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G33/02A01H1/00A01H13/00C12N5/04
Inventor 刘必谦杨锐骆其君王亚军周湘池
Owner 刘必谦
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