Method for culturing pure laver line
A cultivation method and technology of seaweed, applied in botany equipment and methods, cultivation, plant cultivation, etc., can solve the problems that chimeric cells cannot be ruled out, strains are not pure, etc.
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[0012] Embodiments of the present invention are further described in detail below.
[0013] 1. Select fresh immature laver leaves; or partially mature laver leaves on the edge, cut off the edge as much as possible, and keep the immature area in the center. Remove miscellaneous algae, treat with 0.7% KI and 1% penicillin for 10 minutes respectively, and then rinse with sterile sea water several times for aseptic operation. Cut the center area of the treated blade to 1mm 2 Little pieces left and right.
[0014] 2. Use protein extracts from herbivorous shellfish in the intertidal zone of the ocean, such as snails, snails, oysters, etc., with a concentration of 1%, and add NaCl or glucose to make the final concentration of NaCl 1.5mol / L or glucose 2mol / L, will process 1mm in 1 step 2 The small piece is enzymolyzed at 20°C, pay attention to observe the enzymatic hydrolysis, when the 5-6 layers of cells around the leaves are loose, they can be rinsed with sterile sea water for...
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