Cultivation method of transgenic rice sterile line based on wheat pollen lethal gene Ki

Abstract

The invention discloses a cultivation method of a transgenic rice sterile line based on a wheat pollen lethal gene Ki. The cultivation method includes the following steps: A, amplification of the wheat pollen lethal gene Ki; B, obtainment of an mVenus gene expression cassette; C, obtainment of a rice gene expression cassette; D, amplification of a wheat pollen specific promoter Pg47; and E, linkage of all gene expression elements. The step E includes the following steps: firstly, introducing the wheat pollen lethal gene Ki to a pCAMBIA1300 vector; and then linking the mVenus gene expression element, a complete rice EAT1 gene and the wheat pollen specific promoter Pg47 to the pCAMBIA1300 vector. An F1 generation heterozygote follows the Mendelian separation law in a self-fertilization process, and produced progenies contain both the heterozygosis capable of maintaining three linkage genes and the sterile line without fertility.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering genetics and breeding, in particular to a method for cultivating a transgenic rice sterile line based on wheat pollen lethal gene Ki. Background technique [0002] Rice is native to tropical Asia, and after being widely planted in China, it gradually spread to all parts of the world. Rice is the staple food of nearly half of the world's population. In addition to being edible, rice can also be used for wine making, sugar refining and as an industrial raw material. Years of production practice have shown that hybrid rice can generally increase yield by more than 20% compared with conventional rice, so hybrid rice shows great potential for increasing yield. [0003] The development of hybrid rice depends on the cultivation of male sterile lines of hybrid rice. Research on hybrid rice in my country began in the 1960s, and it began to be planted on a large scale in the 1970s. The first-...

AI Technical Summary

Problems solved by technology

In the three-line method, the available parents with good shape are limited, the combination is not free, and the frequency of wild-lost maintenance lines is low

[0004] The genetic difference between the existing sterile lines in production is small, the fertility stability of hybrids is not enough, and the ability to resist adversity is poor

However, in the "two-line method", the fertility of the photothermosensitive male sterile line is controlled by the external temperature, which may easily lead to the self-fertilization of the photothermosensitive male sterile line and the failure of seed production.

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