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Fire-resistant lodging-resistant red lotus type cytoplasmic male sterile series breeding method

A male sterile line, lodging resistance technology, applied in the fields of botanical equipment and methods, plant genetic improvement, climate change adaptation, etc., can solve problems such as fertility instability, achieve mature methods, obvious heterosis, and improve seed production horizontal effect

Inactive Publication Date: 2008-02-06
WUHAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to provide a kind of breeding method of high temperature resistance and lodging resistance red lotus type cytoplasmic male sterile line, the method is easy to implement, easy to operate, and solves the problem of red lotus type cytoplasmic male sterile lines such as Yuetai A in earing. During the flowering stage, high temperature (above 35°C) will cause unstable fertility. At the same time, during the breeding process of the CMS line, attention should be paid to lodging resistance and resistance to diseases and insect pests, so that the Etai A series CMS line has high temperature resistance. , stable sterility, lodging resistance and pest resistance

Method used

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  • Fire-resistant lodging-resistant red lotus type cytoplasmic male sterile series breeding method
  • Fire-resistant lodging-resistant red lotus type cytoplasmic male sterile series breeding method

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Experimental program
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Effect test

Embodiment 1

[0030] Select 6096 and Yuetai B, use artificial detasseling method for crossbreeding, and get F 1 , planting plus generation to get F 2 generation. from F 2 Plant selection was carried out in the generation, and 67 individual plants with short plant type and strong division force were selected, and they were tested and mated with Yuetai A respectively, and 67 combinations were obtained. Under the high temperature conditions of 35°C or 36°C or 37°C or 38°C or 39°C or 40°C, 67 combinations were screened to eliminate the combinations with unstable fertility. For the selected 6 paired combinations, continue to carry out paired backcrossing under high temperature conditions in Wuhan and Hainan, and cycle to the 6th generation, and obtain high temperature resistant, lodging resistant, stable fertility male sterile line Etai A and its other The maintainer line Etai B and its series of CMS and maintainer lines are currently used to test the mating group using the restorer line, and...

Embodiment 2

[0033] (1).Using the artificial emasculation method, the wild-type restorer line "Luohui 988" was artificially emasculated, and the pollen of "Ezhong 5" was inoculated, and the hybrid mating group was bred into 6096 after systematic breeding. It is characterized by compact plant type, medium tillering ability, strong stem, strong lodging resistance, straight leaves, large ears and many grains. The grain is slender, without chalkiness, and has good appearance and eating quality.

[0034] (2). 6096 was used as the female parent for artificial detasseling, Yuetai B was used as the male parent, pollinated, crossed and mated to obtain the F1 generation, and F1 was self-crossed to obtain F 2 Generation, F 2 Separation occurs. from F 2 The isolated strains of the first generation were selected, and 67 single plants with short plant type and strong division force were selected, and they were tested and mated with Yuetai A respectively, and 67 combinations were obtained.

[0035] (...

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Abstract

The invention discloses a breeding method for fireresistant and lodging resistant honglian cytoplasm male sterile lines. The manual detasseling method is utilized to pollinate and hybridize the female parent of 6069 and the male parent of yuetai B to obtain the F1 generation, and the F2 generation is obtained by self-fertilization of F1. The individual plant is chosen from the F2 generation, the short individual plant of strong replication ability is chosen to do testcross with yuetai A to obtain 67 combinations. The backcross is completed in the 67 combinations and corresponding maintenance line individual plant, the sterile anther and farina in didymous plant type are observed, six didymous plant type are chosen to finish backcross under high temperature conditions, wherein the sterile plant in the didymous type has stable sterility, indehiscent anther and 100 percent of anther abortion under high temperature conditions. The didiymous backcross is continuously carried through in the sterile plant of the didymous plants under high temperature conditions, fireresistant novel male sterile line yuetai A is obtained from the sixth generation, the testcross is carried through in the sterile line, thereby obtaining the lodging resistant variety of high quality and high yield utilized in production.

Description

technical field [0001] The present invention relates to the technical field of plant genetics, and more specifically to a method for breeding a high-temperature-resistant and lodging-resistant red lotus-type cytoplasmic male sterile line, a method for using red lotus-type cytoplasmic male sterile lines Yuetai A and 6096 (Luo Hui 988 / Ezhong 5) / Yuetai B were crossed and backcrossed, and a new male sterile line Etai A with high temperature resistance and lodging red lotus type cytoplasm was selected. , the degree of pollen sterility is 100%, the male sterility is stable, the microspores are completely aborted, there is no loose pollen phenomenon, and the lodging resistance is strong. Background technique [0002] Since the realization of the three-line hybrid rice system in my country in 1974, the wild type, red lotus type and BT type are the main types of hybrid rice cytoplasm in the production of hybrid rice in my country. made a huge contribution. However, because the red l...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04A01H5/00
CPCY02A40/138
Inventor 丁毅余金洪
Owner WUHAN UNIV
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