The invention aims at providing rice filling liquid which achieves orderly heading, fast filling, large ears and full grains, early maturing, lodging resistance and increasing of both production and income. The rice filling liquid is prepared from, by weight, 1,000 parts of mixed amino acid mother liquor, 1-2 parts of copper, 1-2 parts of iron, 1-2 parts of manganese, 3-6 parts of boron, 1-2 parts of molybdenum, 0.1-0.2 part of expanding agent, 2-5 parts of cell division agent, 2-5 parts of root promoting, flower protecting and fruit benefiting agent and a proper amount of water. A preparation method comprises the steps that the 1,000 parts of mixed amino acid mother liquor are injected into a reaction still, iron, manganese, boron and molybdenum are dissolved in water with the ratio of iron, manganese, boron and molybdenum to water being 1:10, then the mixture is put into the reaction still, then the expanding agent, the cell division agent and the root promoting, flower protecting and fruit benefiting agent are added, the mixture is heated to 75 DEG C to 80 DEG C, and stirred for 0.5 h to 2 h at the rotating speed of 100 r/min to 150 r/min to be dissolved, heating and heat preservation are carried out, and chelating liquid is added for chelating preparation.