The invention provides an establishment method of an
animal model for evaluating IPV (inactivated poliovirus vaccine) intradermal immunization effectiveness. The establishment method includes the following steps: selecting Yunnan Xishuangbanna small ear pigs belonging to Southern China pigs, with pig ages of 3-5 months and weights of 10-20kg, randomly grouping the pigs, respectively injecting an IPV vaccine through needleless
intradermal injection for the first time, sampling blood after
immunity for 30 days, separating serum, respectively injecting the IPV vaccine through needleless
intradermal injection for the second time, sampling blood after
immunity for 7 days and 30 days, separating the serum, respectively injecting the IPV vaccine through needleless
intradermal injection for the third time, sampling blood after
immunity for 1 month, 6 months, 12 months, 18 months and 24 months, separating the serum, inactivating the serums for 30 minutes at 56 DEG C, conventionally diluting the serums, neutralizing the serums by using Sabin strain I type and II type and Pfizer III type viruses, wherein the dosage of the viruses is 30-300 TCID50 (
tissue culture infectious
dose 50), naturalizing for 3 hours at 35-37 DEG C, adding cells, culturing at 35-36 DEG C for 7 days, and judging a result according to CPE (
Cytopathic Effect), thus establishing the
animal model for evaluating IPV intradermal immunization effectiveness. By adopting the establishment method provided by the invention, a rational
animal model is provided for an intradermal immunization research on vaccines, and a reliable measure is provided for reducing the dosage of
vaccine antigen in the intradermal immunization research.