The invention belongs to the technical field of
biology, and relates to a method for
virus inoculation of maize yellow
mosaic virus based on
aphid mediators and application of the method, the optimum
virus inoculation system of MaYMV is determined by performing MaYMV
virus inoculation on maize seedlings through aphids, and research finds that when five maize aphids are inoculated for 5 days in the maize VE or V1 period, the
virus inoculation efficiency of the MaYMV on maize is the highest; through
virus inoculation, it is clear that after MaYMV infection, the corn can have dwarf and small ear symptoms; high-susceptibility varieties zhu3151 and B73 are used for carrying out MaYMV
virus inoculation, red leaf symptoms appear after infection,
transcriptome analysis is carried out,
gene expression differences of different samples or under different conditions are compared, the change rule of
gene expression is disclosed, and deep understanding of expression
modes and regulatory mechanisms of genes under different conditions is facilitated; the research on the pathogenic mechanism has important significance on
disease prevention and control, and development of a prevention and control method and cultivation of resistant varieties are facilitated.