Establishment method of animal model for ipv intradermal immunity evaluation
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An animal model and intradermal immunization technology, applied in the field of medical biology, can solve the problems of no reports, etc., and achieve the effects of easy standardization, promotion, and simple methods
Active Publication Date: 2016-03-23
INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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[0005] The traditional intramuscular injection route of IPV is used to evaluate the efficacy of Wistar rats as an experimental animal model. There is no report on the efficacy of intradermal immunization route of IPV.
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[0019] The present invention will be further described below in conjunction with specific examples.
[0020] The establishment method of animal model for the evaluation of the effectiveness of IPV intradermal immunity, select 26 Yunnan Banna piglets belonging to the South China type pig breed with a pig age of 3-5 months and a body weight of 10-20kg, half male and half male, through the following steps :
[0021] A. The selected piglets are randomly divided into five groups, i.e. the following three test groups for needle-free intradermal injection: 1 / 3 dose group, 1 / 5 dose group, 1 / 10 dose group, 5 in each group, and intramuscular Injected 6 full-dose control groups and 5 normal saline negative control groups, blood was first collected from each piglet, and the tail of the pig was first disinfected with 75% alcohol in mass concentration, and blood was collected from the tail vein, that is, at the tail of the midline. Insert the needle at an angle of 10-20° above the vein, in...
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Abstract
The invention provides an establishment method of an animal model for evaluating IPV (inactivated poliovirus vaccine) intradermal immunization effectiveness. The establishment method includes the following steps: selecting Yunnan Xishuangbanna small ear pigs belonging to Southern China pigs, with pig ages of 3-5 months and weights of 10-20kg, randomly grouping the pigs, respectively injecting an IPV vaccine through needleless intradermal injection for the first time, sampling blood after immunity for 30 days, separating serum, respectively injecting the IPV vaccine through needleless intradermal injection for the second time, sampling blood after immunity for 7 days and 30 days, separating the serum, respectively injecting the IPV vaccine through needleless intradermal injection for the third time, sampling blood after immunity for 1 month, 6 months, 12 months, 18 months and 24 months, separating the serum, inactivating the serums for 30 minutes at 56 DEG C, conventionally diluting the serums, neutralizing the serums by using Sabin strain I type and II type and Pfizer III type viruses, wherein the dosage of the viruses is 30-300 TCID50 (tissue culture infectious dose 50), naturalizing for 3 hours at 35-37 DEG C, adding cells, culturing at 35-36 DEG C for 7 days, and judging a result according to CPE (Cytopathic Effect), thus establishing the animal model for evaluating IPV intradermal immunization effectiveness. By adopting the establishment method provided by the invention, a rational animal model is provided for an intradermal immunization research on vaccines, and a reliable measure is provided for reducing the dosage of vaccine antigen in the intradermal immunization research.
Description
technical field [0001] The invention relates to a method for establishing an animal model for intradermal safety and immunogenicity evaluation of a vaccine, in particular to a method for establishing a miniature pig animal model for intradermal immune evaluation of an inactivated polio vaccine, and belongs to the field of medical biotechnology. Background technique [0002] Poliomyelitis is an acute infectious disease caused by poliovirus types Ⅰ, Ⅱ, and Ⅲ, which is widely spread and extremely harmful. The clinical symptoms will quickly cause irreversible paralysis, causing flaccid paralysis of muscles, especially limbs, which mostly occurs in children, so it is also called "poliomyelitis". In the mid-to-late 1950s, two scientists in the United States, Dr. Jonas Salk and Dr. Albert Sabin, successively successfully developed inactivated polio vaccine (IPV) and oral polio vaccine (OPV) to prevent and eliminate polio. Yan provides a powerful weapon. Live attenuated polio vacc...
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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00G01N33/15
Inventor 廖国阳孙明波马磊寸怡娜蔡玮周健杨卉娟刘婧张新文戴忠祥代小虎胡文柱姜述德
Owner INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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