Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Establishment method of animal model for evaluating IPV (inactivated poliovirus vaccine) intradermal immunization effectiveness

An animal model, intradermal immunization technology, applied in the field of medical biology, can solve the problem of no report, etc., and achieve the effect of easy standardization, simple method and stable application prospect.

Active Publication Date: 2014-06-25
INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The traditional intramuscular injection route of IPV is used to evaluate the efficacy of Wistar rats as an experimental animal model. There is no report on the efficacy of intradermal immunization route of IPV.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Establishment method of animal model for evaluating IPV (inactivated poliovirus vaccine) intradermal immunization effectiveness

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] The present invention will be further described below in conjunction with specific examples.

[0021] The establishment method of animal model for the evaluation of the effectiveness of IPV intradermal immunity, select 26 Yunnan Banna piglets belonging to the South China type pig breed with a pig age of 3-5 months and a body weight of 10-20kg, half male and half male, through the following steps :

[0022]A. The selected piglets are randomly divided into five groups, i.e. the following three test groups for needle-free intradermal injection: 1 / 3 dose group, 1 / 5 dose group, 1 / 10 dose group, 5 in each group, and intramuscular Injected 6 full-dose control groups and 5 normal saline negative control groups, blood was first collected from each piglet, and the tail of the pig was first disinfected with 75% alcohol in mass concentration, and blood was collected from the tail vein, that is, at the tail of the midline. Insert the needle at an angle of 10-20° above the vein, ins...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an establishment method of an animal model for evaluating IPV (inactivated poliovirus vaccine) intradermal immunization effectiveness. The establishment method includes the following steps: selecting Yunnan Xishuangbanna small ear pigs belonging to Southern China pigs, with pig ages of 3-5 months and weights of 10-20kg, randomly grouping the pigs, respectively injecting an IPV vaccine through needleless intradermal injection for the first time, sampling blood after immunity for 30 days, separating serum, respectively injecting the IPV vaccine through needleless intradermal injection for the second time, sampling blood after immunity for 7 days and 30 days, separating the serum, respectively injecting the IPV vaccine through needleless intradermal injection for the third time, sampling blood after immunity for 1 month, 6 months, 12 months, 18 months and 24 months, separating the serum, inactivating the serums for 30 minutes at 56 DEG C, conventionally diluting the serums, neutralizing the serums by using Sabin strain I type and II type and Pfizer III type viruses, wherein the dosage of the viruses is 30-300 TCID50 (tissue culture infectious dose 50), naturalizing for 3 hours at 35-37 DEG C, adding cells, culturing at 35-36 DEG C for 7 days, and judging a result according to CPE (Cytopathic Effect), thus establishing the animal model for evaluating IPV intradermal immunization effectiveness. By adopting the establishment method provided by the invention, a rational animal model is provided for an intradermal immunization research on vaccines, and a reliable measure is provided for reducing the dosage of vaccine antigen in the intradermal immunization research.

Description

[0001] technical field [0002] The invention relates to a method for establishing an animal model for intradermal safety and immunogenicity evaluation of a vaccine, in particular to a method for establishing a miniature pig animal model for intradermal immune evaluation of an inactivated polio vaccine, and belongs to the field of medical biotechnology. Background technique [0003] Poliomyelitis is an acute infectious disease caused by poliovirus types Ⅰ, Ⅱ, and Ⅲ, which is widely spread and extremely harmful. The clinical symptoms will quickly cause irreversible paralysis, causing flaccid paralysis of muscles, especially limbs, which mostly occurs in children, so it is also called "poliomyelitis". In the mid-to-late 1950s, two scientists in the United States, Dr. Jonas Salk and Dr. Albert Sabin, successively successfully developed inactivated polio vaccine (IPV) and oral polio vaccine (OPV) to prevent and eradicate polio. Polio provides a powerful weapon. Live attenuat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00G01N33/15
Inventor 廖国阳孙明波马磊寸怡娜蔡玮周健杨卉娟刘婧张新文戴忠祥代小虎胡文柱姜述德
Owner INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com