The invention relates to a preparation technology of a strawberry sweetening cultivation medium.The strawberry sweetening cultivation medium is obtained by treating 0.1% urea, folium artemisiae argyi, pericarpium citri reticulatae, bacillus subtilis, crab shells, escargot shells, soybean cakes, banana peel, pine nut shells, 5-aminolevulinic acid, sodium hydrogen sulfite, gibberellin GA4+7, humic acid and choline chloride through the processes such as fermenting, composting and drying.The obtained fertilizer can increase the photosynthetic rate of strawberry leaves, adjust synthesis of chlorophyll, increase the chlorophyll content in the seedling stage, improve the stability of the chlorophyll, improve the photosynthetic efficiency, promote the photosynthesis, promote strawberry tissue differentiation and enlarge pores, the single fruit weight and the total sugar content of strawberries are increased, the soluble sugar content is especially significantly increased, and the total soluble sugar content is increased by 28%-30%.