The invention relates to a method for preparing a strawberry cultivation medium achieving a yield increase. The method comprises the following steps: taking 0.1% urea, folium artemisiae argyi, dried tangerine or orange peel 3, bacillus subtilis, crab shells, escargot shells, bean cakes, banana peel, pine nut shells, brassinolide, paclobutrazol, salicylic acid liquid of 1000 times and Yuhualing, and performing fermentation, decomposition, drying and other processing to obtain the strawberry cultivation medium achieving a yield increase. By inhibiting apical growth of strawberry, increasing effective tillering, accelerating strawberry blooming, increasing percentage of fertile fruits and increasing single strawberry weight, the purpose of yield increase can be achieved, meanwhile the disease resistance of strawberries can be improved, and the yield can be increased by 18-20% on a year-on-year basis.