This invention discloses a rapid method for creating wheat CMS maintainer lines based on
recurrent selection and double haploid technology. The method involves crossing a CMS sterile line with a fertile cytoplasmic restorer line to obtain heterozygous fertile plants. Then, using the restorer line as the recurrent paternal parent, a backcross is performed to convert the
cytoplasm to a fertile
genotype. Double haploid technology is used to rapidly obtain a homozygous DH
population from the backcross progeny. Finally, through testcross identification, DH plants with completely sterile cytoplasmic
cytoplasm are selected, thus obtaining the target
maintainer line with fertile
cytoplasm and a sterile nuclear
genotype. This invention further incorporates
recurrent selection technology for
continuous optimization of the
maintainer line's agronomic traits. This invention integrates
backcrossing, DH technology, and a
recurrent selection system, significantly shortening the breeding cycle. The
maintainer line exhibits homozygosity ≥99%, a pure cytoplasmic background, strong versatility, and applicability to wheat CMS types, providing efficient
technical support for
hybrid wheat breeding.