6 results about "Recurrent selection" patented technology

This invention discloses a rapid method for creating wheat CMS maintainer lines based on recurrent selection and double haploid technology. The method involves crossing a CMS sterile line with a fertile cytoplasmic restorer line to obtain heterozygous fertile plants. Then, using the restorer line as the recurrent paternal parent, a backcross is performed to convert the cytoplasm to a fertile genotype. Double haploid technology is used to rapidly obtain a homozygous DH population from the backcross progeny. Finally, through testcross identification, DH plants with completely sterile cytoplasmic cytoplasm are selected, thus obtaining the target maintainer line with fertile cytoplasm and a sterile nuclear genotype. This invention further incorporates recurrent selection technology for continuous optimization of the maintainer line's agronomic traits. This invention integrates backcrossing, DH technology, and a recurrent selection system, significantly shortening the breeding cycle. The maintainer line exhibits homozygosity ≥99%, a pure cytoplasmic background, strong versatility, and applicability to wheat CMS types, providing efficient technical support for hybrid wheat breeding.

The present invention relates to a breeding system for the production of polyploidy seeds, plants or plant parts in which cycles of meiosis, gamete coordination and selection are used for inter-population improvement of progenitor lines and sexual polyploidy occurs during hybrid production by inducing cloned gamete formation in parents to be hybridized. Mutual recurrent selection can be used to inform the selection of candidate lines that are advanced to a gene editing or genetic modification system, or hybridized and selected to induce clone gamete formation by preventing meiotic recombination and chromosomal subtrahend. Hybridization of parent plants carrying cloned gametes is planned and performed based on the predicted heterosis performance at the polyploidy level. The final product is a homogeneous population of hybrid polyploidy seeds or derivatives thereof, which carries the intact nuclear genome of both parents. In some cases, the methods are used to generate plants that produce seedless fruits.

This invention provides a rice dominant nuclear male sterility expression vector constructed based on the EAT1 gene and its application. The expression vector has... Ubiquitin Promoters and genes for male flower development in rice EAT1 It is formed by operable connections. Specific construction methods include: obtaining rice cDNA and using it as a template, employing... EAT1 Gene-specific primer amplification EAT1 Genes were extracted to obtain PCR product fragments; these PCR product fragments were then ligated into genes containing... Ubiquitin The expression vector is obtained by placing the promoter in a skeletal vector. Transgenic plants obtained using this expression vector exhibit dominant nuclear male sterility, and dominant nuclear male sterile rice lines can be created through hybridization. Recurrent selection breeding using these dominant nuclear male sterile lines can rapidly eliminate non-target materials, efficiently aggregate multiple superior traits and genes, and does not contain transgenic components, effectively improving breeding efficiency and safety.

The invention relates to an SNP (Single Nucleotide Polymorphism) site combination and an application of the SNP site combination in improving the quality character of a recurrent selection group of cotton. The SNP site combination comprises any combination of SNP sites FL-FS-A101, FL-D021, FL-A071, FM-A101, FM-LP-D031, FM-D061, FS-A072, FS-D021, FS-A133 and MSA09. The SNP site combination provided by the invention can be used for cotton quality character prediction and screening identification, fertility site molecular detection is added in cotton whole-genome assisted recurrent selection breeding to assist directional quality genetic improvement of cotton germplasm resources and high-quality cotton breeding, and the SNP site combination has the advantages of high efficiency, accuracy, simplicity and convenience in operation, wide application, low cost and the like, and has a wide application prospect. The method can significantly improve the efficiency and accuracy of cotton fiber quality improvement, has a wide application prospect, shows a detection result in the form of DNA, can detect each tissue and development stage of cotton, is not limited by the environment and seasons, does not have the influence of expression or not, does not need to analyze the length of a fragment, only needs to analyze the variation of SNP, and has a wide application prospect. The method is suitable for rapid, high-throughput and automatic screening.

The present inventions relate to a breeding system for the production of polyploid potato seeds, plants, or plant parts where cycles of meiosis, syngamy, and selection are used for interpopulation improvement of progenitor lines, and sexual polyploidization occurs during hybrid production by inducing clonal gamete formation in the parents that are to be crossed. Reciprocal recurrent selection can be used to inform selection of candidate potato lines that are either advanced to a gene editing or genetic modification system or crossed and selected to induce clonal gamete formation by arresting meiotic recombination and chromosome reduction. Crosses of parent plants bearing clonal gametes are planned and executed based upon predicted heterotic performance at the polyploid level. The final product is a homogeneous population of hybrid polyploid potato seed, or derivative thereof, bearing both parents' complete nuclear genomes. In some instances, the method is used to generate seedless plants.

The present invention relates to a breeding scheme for the production of polyploid seeds, plants, or plant parts in which cycles of meiosis, gamete mating, and selection are used for interpopulation improvement of ancestral lines, and sexual polyploidization occurs during hybrid production by inducing clonal gametogenesis in the parents to be crossed. Reciprocal recurrent selection can be used to inform the selection of candidate lines that are either advanced to gene editing or genetic modification systems or crossed and selected to induce clonal gametogenesis by arresting meiotic recombination and chromosome reduction. Crosses of parent plants with clonal gametes are planned and executed based on predicted heterosis performance at the ploidy level. The end product is a uniform population of hybrid polyploid seeds, or derivatives thereof, that possess the complete nuclear genomes of both parents. In some instances, the method is used to produce plants that produce seedless fruit.