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Method for breeding high oil rape variety by polymerising and sporidiole cultivating technology

A technology of microspore culture and polymerization breeding, which is applied in the directions of botanical equipment and methods, horticultural methods, and applications, can solve the problems of existing technologies and materials of undiscovered high-oil rape varieties, and achieves the improvement of international market competitiveness, The effect of increasing oil content, huge economic and social benefits

Inactive Publication Date: 2005-03-02
河南省农业科学院棉花油料作物研究所
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0007] After investigation and retrieval, no existing technologies and materials for breeding high-oil rapeseed varieties by using aggregation breeding and free microspore culture technology have been found.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A. Select different rapeseed varieties with high oil content, low glucosinolate and other traits; B. Aggregate excellent genes through hybridization, backcrossing, and recurrent selective breeding techniques to obtain hybrids with genes for various excellent traits; C. Use free small Spore culture technology, cultivate a large number of haploid embryoid bodies; D, obtain heritable diploid plants with homozygous genes after chromosome doubling; E, conduct field agronomic traits screening and resistance in many plants Disease identification; F. After harvesting, analyze the quality of the seeds, select homozygous diploid strains with target traits for propagation, and obtain new rapeseed strains with excellent traits such as ultra-high oil content after further screening.

Embodiment 2

[0024] A. Select different rapeseed varieties with high oil content, excellent agronomic traits and other traits; B. Aggregate excellent genes through hybridization, backcrossing, and recurrent selective breeding techniques to obtain hybrids with genes for various excellent traits; C. Use free small Spore culture technology, cultivate a large number of haploid embryoid bodies; D, obtain heritable diploid plants with homozygous genes after chromosome doubling; E, conduct field agronomic traits screening and resistance in many plants Disease identification; F. After harvesting, analyze the quality of the seeds, select homozygous diploid strains with target traits for propagation, and obtain new rapeseed strains with excellent traits such as ultra-high oil content after further screening.

Embodiment 3

[0026] A. Select different rapeseed varieties with high oil content, strong disease resistance and other traits; B. Aggregate excellent genes through hybridization, backcrossing, and recurrent selective breeding techniques to obtain hybrids with various excellent trait genes; C. Use free Microspore culture technology, cultivate a large number of haploid embryoid bodies; D, obtain heritable diploid plants with homozygous genes after chromosome doubling; E, perform field agronomic traits screening and Identification of disease resistance; F. After harvesting, analyze the quality of the seeds, select homozygous diploid lines with target traits for propagation, and obtain new rapeseed lines with excellent traits such as ultra-high oil content after further screening.

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Abstract

The process of breeding high oil content rape variety includes the following steps: selecting rape varieties with high oil content, low thioglycoside content, excellent agricultural characteristic and strong disease resistance; hybridization, backcross and recurrent selection to select excellent comprehensive gene and obtain heterozygote; free microspore culturing to obtain great amount of monoploid embryoid; doubling chromosome to obtain inheritable diploid plant with homozygous gene; field agricultural characteristic screening and disease resistance identification; harvesting and seed quality analysis to obtain homozygous diploid line for propagation; and further screening to obtain the new rape variety with very high oil content. The said technology has been used in culturing new rape variety T057-7 with high oil content.

Description

technical field [0001] The invention relates to a method, in particular to a method for breeding high-oil rapeseed varieties by means of aggregation breeding and microspore culture techniques. Background technique [0002] Rapeseed is an important edible oil and high-protein crop. It is one of the four major oil crops in the world, accounting for more than 40% of all edible vegetable oil. my country is a major producer of rapeseed in the world, ranking first in the world in terms of planting area and output of rapeseed. Therefore, rape occupies an important position in the national economy and people's life. The main product of rapeseed is rapeseed oil, and increasing the oil content of rapeseed is one of the main breeding goals, but the average oil content of rapeseed in my country is generally low, only 35-38%. The volume average is 42%. [0003] At present, domestic and foreign markets have a strong demand for rapeseed. From the perspective of the domestic market, my co...

Claims

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Application Information

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IPC IPC(8): A01G7/00A01H1/02
Inventor 张书芬
Owner 河南省农业科学院棉花油料作物研究所
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