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Microspores are land plant spores that develop into male gametophytes, whereas megaspores develop into female gametophytes. The male gametophyte gives rise to sperm cells, which are used for fertilization of an egg cell to form a zygote.
Methods and compositions for microspore imaging, analysis, culturing, and / or sorting
PCT designated stageWO2026112588A1Microbiological testing/measurementAcquiring/recognising microscopic objectsSporelingImaging processing
Provided herein is a method of imaging and analyzing plant microspores. The method comprises obtaining one or more two-dimensional micrographs of a microspore population, wherein the microspore population has not been labeled; sending the one or more two-dimensional micrographs to an image processing system; segmenting, via the image processing system, the one or more two-dimensional micrographs to distinguish individual microspores, thereby creating a segmented population of microspores; obtaining one or more z-stacks of the population of segmented microspores or the population of microspores to be segmented; and using one or more of pixel intensity information and intensity signal processing within the one or more z-stacks of segmented cells to find nuclear edges and nuclear geometry and determining whether cells are uninucleate or binucleate based on the nuclear edges and nuclear geometry. Also provided herein is a method of imaging and analyzing plant microspores comprising determining microspore viability.
Owner:PIONEER HI BREED INTERNATIONAL INC