Breeding design and identification method for short-haulm compact type cole suitable for mechanized harvest

A compact, rapeseed technology, applied in botany equipment and methods, plant genetic improvement, application, etc., can solve problems such as high planting costs

Inactive Publication Date: 2008-10-29
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Use this breeding method to breed rapeseed varieties that are suitable for high-density planting and m

Method used

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  • Breeding design and identification method for short-haulm compact type cole suitable for mechanized harvest
  • Breeding design and identification method for short-haulm compact type cole suitable for mechanized harvest
  • Breeding design and identification method for short-haulm compact type cole suitable for mechanized harvest

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1: Breeding design and selection of dwarf compact rapeseed

[0062] (1) Hybridization test: Brassica oleracea 5148-2 bred by Huazhong Agricultural University (this strain was preserved in the China Center for Type Culture Collection on January 18, 2006, the preservation number is CCTCC-P200601, and the patent application number is: 200610018257.5) as the female parent, the synthetic yellow rapeseed DH line YN90-1016 provided by Professor G Rakow of Canada [see literature: Dargl J Somers et al. Identification of molecular markers associated with linoleic acid desaturation in Brassica napus. Theoretical and Applied Genetics, 1998, 96(6-7): 897-903; Dargl JSomers, et al. Identification of a major gene and RAPD markers for yellow seed coat color in Brassica napus. Genome, 2001, 44: 1077-1082] for the male parent to cross to obtain hybrids f 1 . Parents and hybrid F 1 Planted in the field, hybrid F 1 Plant about 30 plants (2 rows).

[0063] (2) Establish a doubl...

Embodiment 2

[0065] Example 2: Identification of Dwarf Compact Rapeseed

[0066](1) Morphological identification: For the identification method, refer to the records in the aforementioned "Summary of the Invention". Due to the compact plant type, short stem, short main inflorescence and branches in clusters, the flowering period is concentrated, the pod layer is tight, and the maturity period is consistent (attached figure 2 ), and common rapeseed (attached image 3 ) is very distinct. Therefore, plant type and morphological traits are the characteristic traits to identify dwarf compact rapeseed and its derived progeny, and morphological traits can be used to identify dwarf compact rapeseed and its derived progeny.

[0067] (2) Molecular marker identification

[0068] ①The rapeseed varieties used for molecular marker identification are from the publicly reported and applied rapeseed varieties in China, mainly including: Zheshuang No. 3, Huashuang No. 3, Huyou No. 17, Zhongshuang No. 7,...

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Abstract

The invention pertains to the field of rape breeding and discloses a method for seed selection and identification of a novel dwarf compact type rape adapting to high-density planting and mechanized harvesting. The rape of Variety 5148-2 with apreservation number of CCTCC-P200601 that is bred by Huazhong Agricultural University and yellow-seed rape of DH series YN90-1016 that is synthesized artificially in Canada are taken as seed-parents and crossbred to obtain F1. In inflorescence, the main anthotaxy of an F1 plant and the flower buds with a size ranging from 2.8mm to 3.8mm at the upper branches of the P1 plant are selected, and then chromosome doubling with colchicine and microspore culture are carried out successively to obtain the segregation populations of double haploid. Filed phenotypic characteristic identification are applied for years in various spots to the segregation populations; variant rape materials with five major traits, namely, stem length, branch trait, length of anthotaxy, quantity of silique and growth period differing from those of the common rape and dwarf, compact plant, smallish and acervate branches and main anthotaxy, concentrative florescence and compact pod bearing layers phenotypes are selected to obtain the novel dwarf compact type rape adapting to mechanized harvesting. The invention also discloses a method for molecular identification.

Description

technical field [0001] The invention belongs to the technical field of rapeseed breeding, and in particular relates to a method for breeding design, screening and identification of dwarf compact rapeseed suitable for mechanized harvesting. Background technique [0002] With the development of the economy, the labor force and related cultivation and management costs for crop planting are increasing day by day. Breeding new varieties with high yield and high quality, especially suitable for mechanized harvesting, is one aspect of reducing production costs and improving economic benefits. Developing new The cultivation method etc. is another aspect of enhancing economic benefits. Obviously, cultivating ideal new varieties of crops is the first choice to increase crop yield and income and promote the development of agricultural production, and the cultivation of new varieties is closely related to reasonable breeding methods and the discovery and utilization of specific genetic ...

Claims

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Application Information

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IPC IPC(8): A01H1/04A01H1/02A01H1/08C12Q1/68
Inventor 傅廷栋涂金星马朝芝沈金雄易斌文静李新华漆丽萍杨军刘志文
Owner HUAZHONG AGRI UNIV
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