Ploidy appraising method of isolated microspore regeneration plant strain of non-heading Chinese cabbage
A technology for regenerating plants and head cabbage, which is applied in the directions of instruments, fluorescence/phosphorescence, and analytical materials, etc., to achieve the effects of simple sample preparation, high resolution and accuracy, and fast testing speed.
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[0024] A method for identifying the ploidy of non-heading cabbage free microspore regeneration plants provided by the present invention, comprising:
[0025] 1. Materials and methods: Free microspore culture was carried out on the excellent non-heading Chinese cabbage hybrid 'Sulu' (Geng Jianfeng, 2007), and 156 microspore regenerated plants were obtained. The material used was 'Sulu', which was sold in the market; in Nanjing The Cytogenetics Workstation of the College of Horticulture of the Agricultural University adopts the Epics XL flow cytometer produced by Beckman Coulter Company of the United States to conduct flow cytometry ploidy analysis on the test tube seedlings. The applied analysis software is EXPO32ADCsoftware.
[0026] After the microspore regenerated plants bolted and flowered, the adult morphological identification of all regenerated seedlings was carried out. Haploid plants have slender branches, many branches, small flower buds, petals and anthers, no polle...
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