Method for assist-breeding low erucic acid, low sulfuric glucoside cabbage type rape self-incompatible line with microspore cultivation and SSR making

A technology for culturing Brassica napus and microspores, which is applied in the directions of botanical equipment and methods, applications, angiosperms/flowering plants, etc., can solve the problems of long breeding cycle and low efficiency, save manpower and avoid self-crossing decline. Effect

Inactive Publication Date: 2008-08-27
HUAZHONG AGRI UNIV
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  • Application Information

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Problems solved by technology

In order to solve the problems of long breeding cycle and low efficiency of self-incompatible lines of Brassic

Method used

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  • Method for assist-breeding low erucic acid, low sulfuric glucoside cabbage type rape self-incompatible line with microspore cultivation and SSR making
  • Method for assist-breeding low erucic acid, low sulfuric glucoside cabbage type rape self-incompatible line with microspore cultivation and SSR making
  • Method for assist-breeding low erucic acid, low sulfuric glucoside cabbage type rape self-incompatible line with microspore cultivation and SSR making

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Embodiment 1

[0020] 1. Brassica napus hybrid F 1 the acquisition

[0021] Brassica napus self-incompatibility line S-1300 (Ma Chaozhi et al., Breeding of Brassica napus double-low self-incompatibility line, Journal of Huazhong Agricultural University, 1998, 17(3): 211-213) as the female This, and the male parent, Brassica napus variety 04P63wai-52 (this strain was preserved in the Chinese Type Culture Collection Center (English abbreviation: CCTCC) in Wuhan University, Wuhan City, Hubei Province on September 20, 2007, and the preservation number is F 1 seed.

[0022] 2. Brassica napus hybrid F 1 microspore culture

[0023] Will F 1 The seeds were planted under natural conditions (the field strain number was 05-9-1515), and F 1 plants. For the microspore culture method, refer to the method reported by Yu Fengqun et al. Specifically: when F 1 When the plant blooms the third flower, select three individual plants and number them 05-9-1515-1, 05-9-1515-2 and 05-9-1515-3 respectively. ...

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Abstract

The invention belongs to the technology field of the cole breeding, in particular to the method of the seed selection of the Brassica napus self incompatible line with low erucidic acid and sulfuric glucoside utilizing microspore culture and SSR marker assistance. The method is characterized in selecting the Brassica napus self incompatible line S-1300 as the female parent and maintenance line 04P63 External-52 as the male parent, crossbreeding and obtaining F1, microspore culture F1 and obtaining double haploid (DH) self incompatible line; further screening and obtaining the DH material of the Brassica napus self incompatible line with low erucidic acid and sulfuric glucoside; analyzing the variation of the self incompatible double haploid (DH) line by using the SSR markers, studying six major traits of the self incompatible double haploid (DH) line including the plant height, the first branch number, the first branch silique number, main inflorescence length, main inflorescence silique number and yield per plant; screening the double-low Brassica napus self incompatible double haploid (DH) line superior to the S-1300 in production, related trait and general combining ability. The seed selection provides an easy, rapid and effective method of the double-low Brassica napus inbred incompatible double haploid (DH) line.

Description

technical field [0001] The invention belongs to the technical field of rapeseed breeding, and in particular relates to a method for assisting in breeding low erucic acid and low glucosinolate brassica napus self-incompatibility lines by using microspore culture and SSR markers. Background technique [0002] The utilization of heterosis is the main way to increase the yield of rapeseed, and the selection of dominant hybrid combinations is one of the prerequisites for the utilization of heterosis. Practice has proved that the performance of hybrids is mainly determined by the traits of hybrid parents. The selection of hybrid parents is the key to the preparation of hybrid combinations. Breeding excellent self-incompatible lines can improve the yield and stress resistance of self-incompatible line hybrids. Sex matters. Although self-incompatibility hybrids have the advantages of short breeding cycle and high seed production yield, it is very difficult to improve self-incompati...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04A01H5/00C12N15/65
Inventor 马朝芝王同华张幸果娄延脂王传富
Owner HUAZHONG AGRI UNIV
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