Simplified efficient culture method for brassica napus microspores
A technology of Brassica napus and its cultivation method, which is applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of cumbersome cultivation process, low efficiency and high cost of Brassica napus microspores, and achieve the reduction of test land and reduction of The effect of cost and success rate improvement
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[0045] In order to better illustrate the present invention, examples are given as follows: the improved Brassica napus microspore simplification and high-efficiency culture technology is realized in the following ways:
[0046] In Qinghai Academy of Agricultural Sciences, Xining City, Qinghai Province, during the budding period, in the morning or afternoon when the temperature was at 20°C, all 2.8mm-3.5mm single-nuclei on each individual plant of 31005 (H155×Qva) were repeatedly selected. Put 40 flower buds into a 50mL centrifuge tube filled with crushed ice, put them in an ice box and bring them back to the laboratory. Remove the crushed ice, pour 30mL 84 disinfectant into a 50mL centrifuge tube, close the lid, and sterilize for 10 minutes, during which time shake repeatedly up and down to completely sterilize the 84 disinfectant, flower buds and tube walls. Transfer the sterilized flower buds into another sterile 50mL centrifuge tube in the ultra-clean workbench, and wash 3 ...
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