Simplified efficient culture method for brassica napus microspores

A technology of Brassica napus and its cultivation method, which is applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of cumbersome cultivation process, low efficiency and high cost of Brassica napus microspores, and achieve the reduction of test land and reduction of The effect of cost and success rate improvement

Inactive Publication Date: 2011-10-19
HENAN ACAD OF AGRI SCI
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  • Abstract
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Problems solved by technology

[0005] From the above microspore culture process, it can be seen that the microspore cultur

Method used

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  • Simplified efficient culture method for brassica napus microspores
  • Simplified efficient culture method for brassica napus microspores
  • Simplified efficient culture method for brassica napus microspores

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Embodiment Construction

[0045] In order to better illustrate the present invention, examples are given as follows: the improved Brassica napus microspore simplification and high-efficiency culture technology is realized in the following ways:

[0046] In Qinghai Academy of Agricultural Sciences, Xining City, Qinghai Province, during the budding period, in the morning or afternoon when the temperature was at 20°C, all 2.8mm-3.5mm single-nuclei on each individual plant of 31005 (H155×Qva) were repeatedly selected. Put 40 flower buds into a 50mL centrifuge tube filled with crushed ice, put them in an ice box and bring them back to the laboratory. Remove the crushed ice, pour 30mL 84 disinfectant into a 50mL centrifuge tube, close the lid, and sterilize for 10 minutes, during which time shake repeatedly up and down to completely sterilize the 84 disinfectant, flower buds and tube walls. Transfer the sterilized flower buds into another sterile 50mL centrifuge tube in the ultra-clean workbench, and wash 3 ...

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Abstract

The invention relates to a rape microspore culture method and discloses a simplified efficient culture method for brassica napus microspores, belonging to a new plant variety breeding method. Aiming at the problems in conventional brassica napus microspore culture, the technical aspects of sampling period, time and mode, an extracting solution, a culture method, a doubling method, long-distance sterile transportation and the like are respectively improved, so that the efficiency of the brassica napus microspore culture is greatly improved, the culture method and the culture program are simplified, the culture cost is reduced, and the method disclosed by the invention is convenient for popularization and application.

Description

technical field [0001] The invention relates to a method for cultivating rape microspores, in particular to a simplified and high-efficiency culturing method for brassica napus microspores, which belongs to a new method for cultivating new varieties of plants. Background technique [0002] Brassica napus is the main oil crop in my country, and its genetic breeding, genomics research, metabolomics research, germplasm resource innovation, cytogenetics research and molecular genetics research are very active. Rapeseed microspore culture in vitro has a very wide range of applications in the above fields. [0003] Lichter used the free microspore culture of Brassica napus to obtain regenerated plants in 1982, and then scholars from various countries conducted extensive research on many factors affecting the culture of rape microspores and their seedlings, which made the microspore culture technology of rapeseed more simplified and more efficient. High and easy to operate. Chine...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 文雁成张书芬王建平朱家成赵磊
Owner HENAN ACAD OF AGRI SCI
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