Breeding process of obtaining radish haploidy

A single and ploidy radish technology, applied in the field of plant genetics and breeding, can solve the problems of low embryo emergence rate and limited number of regenerated plants, and achieve the effects of improving selection efficiency, speeding up the breeding process, and shortening the time

Inactive Publication Date: 2008-10-15
NANJING AGRICULTURAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

On the whole, the problems that appear concentratedly are the low embryonic rate in radish free microspore culture research, and the limited number of regenerated strains (Takahata, Y.; Komatsu, Hisashi.; Kaizuma, Norihiko. Microspore culture of radish (Raphanus sativus .L): influence of genotype and culture conditions on embryogenesis. Plant Cell Report, 16:163~166; 1996; Zhou Zhiguo, Gong Yiqin, Wang Xiaowu, Liu Liwang, Zhang Yanguo. Research on the induction of free microspores of different radish varieties and the optimization of the culture system. Northwest China Acta Bot, 2007, 27(1): 0033~0038)

Method used

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  • Breeding process of obtaining radish haploidy
  • Breeding process of obtaining radish haploidy

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Embodiment Construction

[0025] (1) Culture of microspores

[0026]Donor plants from radish material NAU-xxxs-03 (Wang Wei, Liu Liwang, Gong Yiqin, et al. Analysis of enzyme activities related to carbohydrate and sucrose metabolism in the process of radish fleshy root swelling. Acta Horticultural Science, 2007, 34(5): 1313-1316) Select flower buds of about 1.5-5.0 mm above, use the petal-to-drug ratio (petal length / anther length), select flower buds with the same length as the petal-to-drug ratio or slightly shorter than the anther, and disinfect the buds with 75% alcohol for 60 seconds, and then use 8 % sodium hypochlorite solution surface disinfection for 15 minutes, rinsed with sterile water 3 times. Put the flower buds in a sterilized mortar to add 0.015% Ca by mass 2+ , pH 5.8 B 5 -13 liquid medium (B 5 +13% sucrose, B 5 See Gamborg, O.L.; Miller, R.A.; Ojima, K. Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res. 50: 151-158; 1968) as the washing solution, and f...

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Abstract

The invention discloses a rapid breeding method of monoploid radish and belongs to the field of plant genetics and breeding. The method comprises the following steps: culturing radish microspores that are subjected to heat treatment at 32.5 DEG C in a constant-temperature incubator for 48 hours to obtain cotyledonous embryoids, culturing the cotyledonous embryoids for 5-7 days at 25 DEG C under 4000Lx light illumination (16 h/d), transferring to a subculture medium for germination until the embryoids turn green, transferring to a rooting medium for plant regeneration, and identifying the regenerated plants by root tip cell chromosome count method or flow cytometry. The method is successful in the culture of regenerated plant of monoploid radish and increases the induction rate of embryoid from microspore up to 17.03/105. The self-bred line of monoploid plant of radish cultured by microspore culture method is obtained by chromosome doubling and is used for genetic improvement and new variety breeding of radish.

Description

technical field [0001] The invention relates to a breeding method for rapidly obtaining radish haploids by using free microspore culture technology, belonging to the field of plant genetics and breeding. Background technique [0002] Free microspore culture is one of the effective ways for crops to produce haploids, and the doubling haploid (DH) population formed by spontaneous or artificially induced doubling of haploids plays an important role in the research of crop genetics and molecular breeding (Oleszczuk, S .; Sowa, S.; Zimny, J. Directembryogenesis and green plant regeneration from isolated microspores of hexaploid triticale (× Triticosescale Wittmack) cv. Bogo. Plant Cell Rep, 22:885-893; 2004). (1) Chromosome doubling of haploid plants obtained by microspore culture can obtain stable homozygotes in one generation, greatly shorten the time of parental homozygotes, speed up the breeding process, and improve the selection efficiency of the required gene combination. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/08A01H4/00A01G31/00C12Q1/68C12N5/04
Inventor 龚义勤赵艳玲柳李旺周治国张翠萍
Owner NANJING AGRICULTURAL UNIVERSITY
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