Breeding process of obtaining radish haploidy
A radish haploid technology, applied in the field of plant genetics and breeding, can solve the problems of limited number of regenerated plants and low embryo rate, and achieve the effects of speeding up the breeding process, improving the selection efficiency and expanding the scope.
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[0025] (1) Culture of microspores
[0026]Donor plants from radish material NAU-xxxs-03 (Wang Wei, Liu Liwang, Gong Yiqin, et al. Analysis of enzyme activities related to carbohydrate and sucrose metabolism in the process of radish fleshy root swelling. Acta Horticultural Science, 2007, 34(5): 1313-1316) Select flower buds of about 1.5-5.0 mm above, use the petal-to-drug ratio (petal length / anther length), select flower buds with the same length as the petal-to-drug ratio or slightly shorter than the anther, and disinfect the buds with 75% alcohol for 60 seconds, and then use 8 % sodium hypochlorite solution surface disinfection for 15 minutes, rinsed with sterile water 3 times. Put the flower buds in a sterilized mortar to add 0.015% Ca by mass 2+ , pH 5.8 B 5 -13 liquid medium (B 5 +13% sucrose, B 5 See Gamborg, O.L.; Miller, R.A.; Ojima, K. Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res. 50: 151-158; 1968) as the washing solution, and f...
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