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Method for improving selection efficiency in hybrid rice breeding

A technology of hybrid rice and high efficiency, which is applied in the field of hybrid rice breeding and selection, can solve the problems that have not been reported, and achieve the effect of improving selection efficiency and reducing blindness

Inactive Publication Date: 2009-07-22
重庆市农业科学院生物技术研究中心 +1
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  • Method for improving selection efficiency in hybrid rice breeding

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Embodiment Construction

[0021] The present invention is described further below:

[0022] F 1 generation seed, F 1 F 2 Substitute seeds; Planting F 2 A kinship selection population was established from generation seeds, and a total of 11,500 lines were planted. Taking Kehui 675 as a control, kinship selection (morphological selection) was carried out with multiple agronomic traits such as plant height, ear length, and leaf color as selection indicators, and obtained 1309 strains were selected, the DNA of the selected strains was extracted, and the molecular markers RM254, RM144, RM224, RM139, RM456, RM536 and MRG4766 closely linked with the broad-spectrum blast resistance gene Pi-1 were used to recover 675 and Differential selection was carried out between LAC23, and molecular markers RM224 and RM144 with large differences were obtained in the molecular electrophoresis pattern. Using these two markers, LAC23 was used as a control to carry out molecular selection on the morphologically selected str...

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Abstract

The invention discloses a method for improving the culture selection efficiency of the hybrid rice. The method is characterized by comprising the following steps: selecting a good plant line in the field with the reference to restorer line Kehui 675: selecting the restorer line Kehui 675 with a great restoring ability as a female parent and the LAC 23 which contains a rice blast resistant gene Pi-1 and comes from Libya as a male parent to obtain a F1 hybrid seed through hybridization; carrying out the inbred of the inbreeding of the F1 hybrid seed to obtain a F2 generation seed; establishing a parent tropic selection group with the reference to the F2 generation seed; harvesting the selected parent tropic individual plant and the Kehui 675 seed; making use of the differential molecular mark between the Kehui 675 and the LAC23 to select the plant line containing the rice blast resistant gene Pi-1 with the reference to the mark of the LAC23; and through the cross selection of parental pair comparison and pedigree seed selection, obtaining the F6 generation and selecting the public plant line with a strong restoring ability and rice blast resistance. The method has the advantages of improving the selection efficiency, polymerizing a plurality of advantageous genes, and creating new excellent resources.

Description

technical field [0001] The invention relates to a hybrid rice breeding selection method, in particular to a method for improving hybrid rice breeding selection efficiency. Background technique [0002] Since the potential of many traditional agricultural technologies has been brought to the extreme and cannot meet the needs of sustainable economic development, the advantages and importance of innovation in rice breeding theory and methods are increasingly revealed. Since the 1990s, the innovation of rice breeding theory and methods has entered a period of rapid development, and has made remarkable achievements in both basic research and application development. As a key technology in the 21st century, the innovation of rice breeding theory and method has been highly valued and supported by countries all over the world. They are competing to formulate development plans. The government and business circles have invested heavily. The country implements very generous industrial ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
Inventor 赵正武李贤勇雷开荣李经勇陈旭蒋晓英
Owner 重庆市农业科学院生物技术研究中心
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