The invention discloses a rice hybrid seed production method. The rice hybrid seed production method comprises the steps that firstly, a base sequence, a WA352 gene sequence and an exogenous reporter gene of a mitochondrial signal peptide are integrated into a wild rice chromosome, a transgenic sterile strain containing the base sequence, the WA352 gene sequence and the exogenous reporter gene of the mitochondrial signal peptide is obtained, and the base sequence, the WA352 gene sequence and the exogenous reporter gene of the mitochondrial signal peptide are linked for expression; secondly, the transgenic sterile strain as a female parent is hybridized with a restorer line as a male parent, F1 hybrid seeds are obtained, the F1 hybrid seeds with the exogenous reporter gene are sterile seeds, and the remaining F1 hybrid seeds are normal hybrid rice seeds and are used for production. The method breaks through the confine of thought of the sterile gene and the restoring gene, male abortion is stable and thorough without affection of environmental conditions, the rate of outcrossing seed-setting is high, the seeds are an ideal material for recurrent selection, and more convenience is brought to the germplasm innovation process.