Methods of producing haploid and doubled haploid oil palms

A technology of double haploid and haploid, applied in botany equipment and methods, biochemical equipment and methods, plant products, etc.

Inactive Publication Date: 2010-03-31
SUMATRA BIOSCI PTE
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Oil palm is primarily an outbred species, but unlike maize, which produces both male and female flowers on the same plant, each oil palm plant produces only male or female flowers at any one time, so a tree cannot simply self-pollinate

Method used

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  • Methods of producing haploid and doubled haploid oil palms
  • Methods of producing haploid and doubled haploid oil palms
  • Methods of producing haploid and doubled haploid oil palms

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Embodiment

[0092] The following examples are used to further illustrate and explain the present invention, but should not be construed as limiting the present invention in any way. It shows the application of the invention to oil palm, but it is equally applicable to dates.

[0093] All references are incorporated herein by reference.

[0094] (1a) Seed treatment

[0095] 1. Remove the mesocarp of oil palm seeds by mechanical means, and air-dry the seeds at room temperature for 24 hours, and then air-dry them in an air-conditioned room at 25°C for 24 hours, so that the moisture content of the seeds reaches 15-18%. The seeds are then stored, usually in plastic bags or trays in an air-conditioned room (25°C) for 1-3 months (although seeds can be stored this way for up to 1 year).

[0096] 2. Soak the seeds for 3 days to increase their water content to 18-20%, and then heat-treat them in plastic bags or plates at 38-40°C for 40-60 days.

[0097] 3. After heating, the seeds were soaked ...

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Abstract

The present invention relates to haploid oil palm plants and homozygous doubled haploid oil palm plants. The invention also relates to methods for producing and selecting haploid and doubled haploid plants. More particularly, but not exclusively, the method may be used for selecting haploid and doubled haploid oil palm plants. Haploid and doubled haploid plants are selected by a large-scale screening based on a combination of the phenotype with the use of molecular methods combined with flow cytometry techniques to identify haploid and doubled haploid plants. More particularly, a method for selecting haploid and doubled haploid plants is described comprising: (a) germinating seeds; (b) selecting seedlings with atypical phenotype; (c) assessing heterozygosity using markers; (d) isolating cells from the seedlings and determining the DNA content of the cells; and (e) isolating and purifying the DNA and using defined molecular markers to characterise the genotype of the plant. The haploidoil palm plants may be used for producing homozygous doubled haploid oil palms: doubled haploids may be intercrossed to produce uniform F1 hybrids of superior properties.

Description

field of invention [0001] The present invention relates to haploid palm plants and homozygous double haploid palm plants. The invention also relates to methods of producing and selecting haploid plants and double haploid plants. More specifically, the method can be used to select haploid and homozygous double haploid oil palm and date palm plants, but is not limited thereto. Background of the invention [0002] While plant breeding programs worldwide have made remarkable progress in producing new varieties with increased yields, increased resistance to pests and diseases, and other useful traits, breeding as a whole relies on extensive plant screening to identify new varieties. expected traits. Often, a very large number of progeny obtained by crossing must be grown and evaluated over several years in order to select one or a few plants with a desired combination of traits. [0003] In conventional plant breeding, two parental plants are crossed, the resulting progeny are...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68A01H1/04A01H5/00
CPCA01H1/04
Inventor 彼得·康纳·纳尔逊詹姆斯·马丁·邓瓦尔迈克尔·詹姆斯·威尔金森彼得·道格拉斯·萨瓦里亚·卡尔加里
Owner SUMATRA BIOSCI PTE
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