Method for improving cabbage type rape by use of cabbage

A technology for Brassica napus and Brassica oleracea, which is applied in the directions of plant genetic improvement, botanical equipment and methods, applications, etc., can solve the problems of unstable synthetic materials, poor self-cultivation, and long time required for ploidy stability, etc. The effect of self-trading is simple and easy, and the cycle is short

Inactive Publication Date: 2014-05-07
SOUTHWEST UNIV
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Problems solved by technology

[0003] The traditional method of using cabbage is to hybridize cabbage and Chinese cabbage, and transfer the genetic components of cabbage to Brassica napus through the artificial synthesis of Brassica napus. However, this method will simultaneously introduce the A genome of Chinese cabbage into Brassica napus that needs to be improved. Moreover, the artificial synthetic material produced by this method is extremely unstable, has poor self-fertilization, and takes a long time for its ploidy to stabilize (Robert et al.2007)

Method used

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Embodiment Construction

[0013] The breeding route of the present invention is "hexaploid F1-BC1F1-BC1F2", that is: Brassica napus is used as the female parent, cabbage is used as the male parent, 8 to 12 days after artificial emasculation and pollination, the seed is obtained by embryo rescue and chromosome doubling technology. The inter-hybrid test-tube seedlings were rooted and planted in the field; after the F1 plants flowered, they were used as the female parent to backcross with the Brassica napus parent, and all the harvested seeds were planted to obtain the BC1F1 generation; the pollen fertility of the BC1F1 generation was higher than that of 85% of the individuals whose target traits are significantly better than the parents of Brassica napus are self-crossed. If the target traits are qualitative traits, the target traits of the selected individuals must be the same as those of the Brassica napus parents. If the target traits are quantitative traits, the selected individuals The target traits ...

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Abstract

The invention belongs to the technical field of plant breeding, particularly relates to a method for improving the cabbage type rape by use of cabbage, and aims at providing a new option to the improvement of the cabbage type rape. The method for improving the cabbage type rape by use of cabbage comprises the following steps: (1) obtaining an interspecific hexaploid F1 hybrid; (2) obtaining and screening the hexaploid F1 hybrid and the backcross first-generation BC1F1 of cabbage type rape; (3) screening the BC1F2 generation. The method provided by the invention can be used for improving the cabbage type rape and is simple and easy to implement with short cycle.

Description

technical field [0001] The invention belongs to the technical field of plant breeding, and in particular relates to a method for improving Brassica napus by using cabbage. Background technique [0002] Brassica napus (AACC, 2n=38) is a natural hybrid of Brassica napus (B.rapa, AA, 2n=20) and Brassica oleracea (B.oleracea, CC, 2n=18). Allotetraploid (U1935). Brassica napus has a narrow genetic base compared to the parent species, limiting genetic improvement of Brassica napus. Utilizing parent species resources is an important way to improve existing Brassica napus and broaden its genetic base. There are ten wild types and multiple cultivated varieties of cabbage in nature, which are rich in genetic diversity and have excellent stress resistance (Ellis et al.1999; Mei et al.2011; Mithen and Magrath1992; Ramsey and Ellis1994). There is great potential for Brassica napus to broaden its genetic base. [0003] The traditional method of using cabbage is to hybridize cabbage an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
Inventor 梅家琴钱伟李加纳刘瑶李勤菲丁一娟
Owner SOUTHWEST UNIV
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