Fast breeding method for black spot resistant sweet potato variety
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A technology for anti-black spot and black spot disease, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve the problems of long breeding years, heavy workload and high cost, and achieve simple breeding methods and the number of single plants. Reduced effects of short breeding cycles
Inactive Publication Date: 2015-11-04
CROP RES INST SHANDONG ACAD OF AGRI SCI
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There are certain deficiencies in the traditional hybrid breeding method of sweet potato resistant to black spot disease: firstly, when matching hybrid combinations, more attention should be paid to the yield level and quality traits of the father and mother, and less attention should be paid to the black spot resistance of the parents; The number of F1 generation hybrid single plant populations is relatively large, and more manpower and material resources are required for f...
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Embodiment 1
[0028] 1. Breeding process
[0029] (1) Hybrid combination configuration
[0030] Lushu 7, which is highly resistant to black spot and has high yield potential, was used as the female parent, and Jishu 15, which was resistant to black spot, was used as the male parent to sexually cross, and 3500 F1 hybrid seeds were obtained.
[0031] (2) Single plant screening of F 1 generation sweet potato
[0032] Sow the obtained 3,500 hybrid seeds in the vermiculite powder in the greenhouse, strengthen management after emergence, topping to promote branch growth, and select 3,000 healthy and healthy plants; each individual plant selects 4 growths that are basically the same, 4-5 Branches with internode length (about 15cm); 3 of them were subjected to inoculation induction of black spot conidia (Method A) (induction of black spot conidia refers to: implanting water-washed spores In sand, use a concentration of 1*10 4 individuals / ml black spot conidia suspension, watered and cultured at ...
Embodiment 2
[0046] 1. Breeding process
[0047] (1) Hybrid combination configuration
[0048] Ji 09184 with high resistance to black spot disease was used as the female parent, and Jishu 16 with good resistance to black spot disease was used as the male parent, and 1500 F1 hybrid seeds were obtained by sexual crossing.
[0049] (2) Single plant screening of F 1 generation sweet potato
[0050]Sow the obtained 1,500 hybrid seeds in the vermiculite powder in the greenhouse, strengthen management after emergence, topping to promote branch growth, and select 1,200 well-grown plants. Each individual plant selects 4 growths that are basically the same, 4-5 Branches with an internode length (about 15cm). Among them, 3 branches were implanted in fine sand for inoculation induction of black spot conidia (Method A). The highly susceptible variety Xushu 18 was used as a control. After 6 days, the disease grade was obtained by screening according to Table 1 of Example 1 and obtained a disease grade...
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Abstract
The present invention relates to a breeding method for a black spot resistant sweet potato variety, and belongs to the technical field of hybridization breeding. The method of the present invention comprises steps of: acquiring F1 hybrid true potato seeds, screening single plants of the black spot resistant sweet potatoes, and performing fast breeding. The assessment and selection of the black spot resistant material is performed at the first round so as to eliminate disease-resistless materials; and it is maximally ensured that the black spot resistant single plants in the hybrid cultivars are selected. Given that ''the number of the selected black spot resistant single plants'' is much smaller than ''the number of the single plants that are selected according to the yield level''; so that the number of the single plants that need to be assessed in the field decreases dramatically and the workload is significantly reduced; an experiment of comparison between yields of geographically different locations can be performed at a time and the breeding period can be foreshortened by 2 to 3 years. The breeding method provided by the invention has the characteristics of simple breeding process, short breeding cycle, high accuracy, less blindness and high efficiency. The novel black spot resistant sweet potato variety with good comprehensive properties is bred, and has good economic, social and ecological benefits.
Description
technical field [0001] The invention relates to a rapid breeding method for black spot-resistant sweet potato varieties, which belongs to the technical field of hybrid breeding. Background technique [0002] Sweet potato black spot is the most common disease in sweet potato production in my country. It is widely distributed and occurs heavily in North China, Huanghuaihai Basin, Yangtze River Basin, and southern summer and autumn potatoes. The main cause of block rot and death of potato seedlings. Diseased potatoes contain toxins and cannot be eaten. If livestock eat more by mistake, it can cause poisoning, and severe cases can be fatal. In recent years, the planting area of fresh and high-quality processed sweet potatoes in my country has expanded rapidly, but there is a shortage of sweet potato varieties resistant to black spot disease in production, and black spot disease occurs seriously during storage. The annual loss caused by it accounts for about 10%, and it can re...
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