Method for culturing triploid petunia

A cultivation method and triploid technology, which are applied in the field of horticultural plant genetics and breeding, can solve the problem of no triploid petunia and the like, and achieve the effects of solving the problem of lack of flowering, improving the induction rate, and the method being simple and easy to implement.

Active Publication Date: 2015-11-18
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no public report on the breeding method of triploid petunias (2n=3x=21)

Method used

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  • Method for culturing triploid petunia
  • Method for culturing triploid petunia
  • Method for culturing triploid petunia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 A method for breeding triploid petunia

[0032] 1) Select the diploid petunia Petuniahybrida'Pink' as the male parent, and the diploid petunia Petuniahybrida'RoseHalo' as the female parent, all purchased from Zhejiang Hongyue Flower Co., Ltd., and planted according to conventional techniques;

[0033] 2) The parents enter the flowering period. At 9 am, the temperature in the greenhouse is 28℃. Choose the flower buds on the male parent plants with a length of 2.6mm~3.1mm. Use a micro syringe to prepare the final mass concentration of colchicine 0.2%, ammonia Sulfuryl 30mg.L -1 , Trifluralin 80mg.L -1 , The volume of 10μL mixed medicinal solution was injected into the flower buds and treated once; after the pollen matures, take the pollen grains on a glass slide and stain them with a mass concentration of 1% acetic acid magenta, and measure the pollen content with a micrometer under a 40-fold microscope. The diameter and appearance characteristics of the 2n pollen ar...

Embodiment 2

[0047] Example 2 A method for cultivating triploid petunia

[0048] 1) Select the diploid petunia Petuniahybrida’MERLIN’ as the male parent, and the diploid petunia Petuniahybrida’U-IRIS’ as the female parent, both purchased from Zhejiang Hongyue Flower Co., Ltd. and planted according to conventional techniques;

[0049] 2) The parents enter the flowering period, the temperature in the greenhouse is 31℃ at 10 am, choose the flower buds on the male parent plants with a length of 2.7mm~3.2mm, and use a micro syringe to prepare the final mass concentration of colchicine 0.1%, sulfonamide Ling 20mg.L -1 , Trifluralin 50mg.L -1 , The volume of 12μL mixed medicinal solution was injected into the flower bud, and treated once;

[0050] After the pollen matures, take the pollen grains on a glass slide and stain them with a mass concentration of 1% acetic acid magenta, measure the diameter of the pollen with a micrometer under a 40-fold lens and confirm the 2n pollen based on the appearance ch...

Embodiment 3

[0065] 1) Select the diploid combination with strong hybridization as the parents, among which the male parent is the diploid petunia Petuniahybrida'PURPLE' and the female parent is the diploid petunia Petuniahybrida'HAPPINESS', all purchased from Hongyue, Zhejiang Flower Co., Ltd., planted according to conventional techniques;

[0066] 2) The parents enter the flowering period, the temperature in the greenhouse is controlled at 22 ℃ at 8 am, the upper longitudinal axis length of the male parent plants is selected to be 2.2mm ~ 3.4mm flower buds, and the final mass concentration of colchicine is prepared with deionized water with a micro syringe , Amisulfaline and Trifluralin, in which the final mass concentration of colchicine is 0.5%, and the final mass concentration of Amisulfaline is 50mg.L -1 , The final mass concentration of trifluralin is 100mg.L -1 Inject the mixed medicinal solution into the flower bud, 15μL each time, for 3 consecutive days;

[0067] After the pollen matu...

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Abstract

The invention discloses a method for culturing triploid petunia. The method comprises the following steps: selecting a diploid petunia combination with strong heterosis as a crossing parent, selecting proper flower buds in the flowering period of a male parent, carrying out chemical mutagenesis by adopting mixed liquid of colchicine, oryzalin and trifluralin to generate 2n pollens, pollinating the 2n pollens to a diploid female parent stigma to obtain F1 hybridized seeds, and hybridizing n pollens the male parent of which is not subjected to chemical mutagenesis with the female parent to obtain contrast diploid F1 hybrid seeds. The adult plants developed from the F1 hybridized seeds are compared with the contrast diploid F1 hybridized filial generations in the aspects of agronomic trait and anatomy, and on this basis, the number of chromosomes can be detected so as to finally determine the triploid plant. According to the method disclosed by the invention, the breeding period is short, the operation is convenient, the mutagenesis efficiency is high and the application prospect is good.

Description

Technical field [0001] The invention belongs to the field of genetic breeding of horticultural plants, and particularly relates to a method for breeding triploid petunia. Background technique [0002] Petunia (PetuniahybridaVilm.) is a plant of the genus Solanaceae Petunia, an annual herbaceous flower. Petunia has huge flowers, rich colors, and many flower types. At present, petunia has become the main potted flower and decorative plant in the world. It is widely used in the beautification and greening of courtyards, open-air flower beds and squares. It is called " The king of flower beds in the world". Petunia in my country began to be introduced and cultivated in a small amount in the early 20th century. In the 1980s, new varieties were gradually introduced from Europe and the United States for large-scale planting. Now with economic development and urban construction, the demand for petunia is increasing, and it is now widely planted in China. The high-quality petunia varieti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/08
Inventor 魏跃樊开青陈啸寅颜志明王全智贾思振史红林
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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