43results about How to "Solve the problem of flowering" patented technology

The invention provides a tobacco pollen lyophilization and low-temperature mid-long-term preservation method. The tobacco pollen preservation method comprises the specific steps that: fresh pollen is placed into a pollen storage container; the container is covered and is placed in a cold trap of a lyophilization machine; the pollen is pre-frozen for 2-4h under a temperature of -45 DEG C to -50 DEG C; the pollen is fetched and is paved on a drying plate or a material box of the lyophilization machine; the pollen is lyophilized for 8-12h in the lyophilization machine under a temperature of -40 DEG C to -50 DEG C; the pollen is sealed in a pollen storage bottle or pollen storage bag; the pollen is placed in a freezer under a temperature of -4 DEG C to -18 DEG C, and can be preserved for a long time in low temperature till a pollination period; the pollen is fetched, the sealing is removed, and pollen activity is recovered under room temperature. With the method provided by the invention, a problem of flowering period missing during a tobacco hybrid seed production process is solved. The male parent is planted in advance, and the plantation of female parent is arranged according to the male parent collection amount. Therefore, blind plantation of parents and soil resource waste are reduced, pollination rate is greatly improved, and cost is greatly reduced.

The invention provides a preservation method for paternal pollens in the capsicum hybrid production. In the capsicum hybrid production, male parents and female parents are planted in a time staggering and allopatry mode, and the pollens of the male parents, which are concentratedly stored in liquid nitrogen, are transported to female parent planting sites for the usage during pollination. According to the invention, the flowering asynchronism of the male parents and the female parents in the capsicum hybrid production is thoroughly solved, so the simultaneous and sympatric appearance of capsicum at the seed production male parents and the seed production female parents is avoided, and the loss of the parent of the male parents can be effectively prevented; and superfluous pollens in the seed production can be stored, so the stored pollens can be used to cover the shortage when the amount of the pollens of the male parents is not enough.

The invention provides a method for quickly preparing pear flower powder. The method comprises the following steps of: (A) pear flower bud collection: collecting balloon-shaped pear flower buds in the large bud period of pear trees, wrapping the collected pear flower buds by gauzes in rainy weather, dehydrating the collected pear flower buds in a drying machine or a washing machine, and removing rainwater on the flower buds; (B) collecting anthers; and (C) high-temperature baking: baking for 28 to 32 minutes at the temperature of 58 to 60 DEG C. According to the method provided by the invention, the pear flower powder can be prepared within 30 minutes. According to a conventional method, the pear flower powder needs to be prepared within 48 hours. With the method, the management cost of labor is saved, and the preparation efficiency of flower powder is improved. The problem of flowering asynchronism between pear varieties namely between a pollination variety and a cultivated variety caused by poor external natural environment in the pear artificial pollination process is solved. The loss of a pear farmer is reduced, and the economic benefit of the pear farmer is improved.

The invention discloses a method for culturing triploid petunia. The method comprises the following steps: selecting a diploid petunia combination with strong heterosis as a crossing parent, selecting proper flower buds in the flowering period of a male parent, carrying out chemical mutagenesis by adopting mixed liquid of colchicine, oryzalin and trifluralin to generate 2n pollens, pollinating the 2n pollens to a diploid female parent stigma to obtain F1 hybridized seeds, and hybridizing n pollens the male parent of which is not subjected to chemical mutagenesis with the female parent to obtain contrast diploid F1 hybrid seeds. The adult plants developed from the F1 hybridized seeds are compared with the contrast diploid F1 hybridized filial generations in the aspects of agronomic trait and anatomy, and on this basis, the number of chromosomes can be detected so as to finally determine the triploid plant. According to the method disclosed by the invention, the breeding period is short, the operation is convenient, the mutagenesis efficiency is high and the application prospect is good.

The invention discloses a soybean GmFTL4 protein and a soybean GmFTL6 protein, with an amino acid sequence shown as SEQ ID No.1 and SEQ ID No.2 respectively, or an equally functional amino acid sequence formed through replacement, deletion, or addition of one or a plurality of amino acids of SEQ ID No.1 or SEQ ID No.2. The expression of soybean anthesis genes GmFTL4 and GmFTL6 can obviously promote a plant (Arabidopis thaliana) to blossom, and shorten an anthesis time and a fertility stage. In addition, the invention can be applied to solve problems of flowering asynchronism in crossbreeding,fertility stage control of various crops, vegetables, fruits and flowers, photoperiod sensitivity, and introduction.

The invention provides a pollen collecting method for tobacco hybrid seed production, and belongs to the technical field of the tobacco improved variety breeding. The method comprises the following steps: picking flowers that the growth stages are consistent, the corollas will be opened, and the top ends are light red and inflated in the male parent field, threshing by using an anther thresher, separating the anther from the impurities, such as filaments, stigmas and petals by using 20 meshes of a fine sieve, collecting the anther, placing in a constant temperature drying cabinet and drying, taking the anthers after the anther is completely cracked and the pollen is completely dispersed, separating the pollen from pollen sac by using 60 meshes of a sample sieve, collecting the pollen, loading in a wild-mouth bottle, and placing in a dryer loaded with a drying agent, storing in a freezer for future use in low temperature. The pollen collecting method is capable of reducing the male and female parent planting proportion, solving the problem that the flowering phases of the male and female parents in the tobacco hybrid seed production are asynchronous, reducing the labor force for the tobacco hybrid seed production, reducing the cost of the hybrid seed production, and improving the efficiency of the hybrid seed production.

The invention discloses a gladiolus crossbreeding method using a sunlight greenhouse. Seeds are sown within the mid-to-end of August and the first ten-day period of September when the outdoor highest temperature in not higher than 29 DEG C; cold protection, heat preservation and ventilation are carried out after seedball treatment, soil treatment, soil preparation, sowing and hauling are carried out; daily management refers to moisture management, humiture management and topdressing; artificial pollination is carried out after flowering at the last ten-day period of November in the same year; and seeds are mature 5-6 weeks later. The gladiolus crossbreeding method can greatly delay the flowering phase, effectively solves the inconsistent flowering phase problems of different varieties, and simultaneously provides enough artificial pollination time for crossbreeding work, reduces workload because bagging is not needed before and after flowering, the operation of stamen removal is only carried out before pollen sacs break, and bagging is also not needed before and after pollination, and greatly increases maturing rate, wherein the maturing rate is over 80% and each capsule contains over 60 grains. By adopting the sunlight greenhouse for crossbreeding, the sowing time is delayed, and seasons with high temperature, high humidity and abundant rainwater are avoided, fruit setting is favored and insect pest is prevented.

The invention discloses a protein related to a plant flowering period as well as a coding gene and application of the protein. According to the technical scheme, the protein derived from soybeans is applied to regulation and control of plant flowering or shortening of the plant flowering period, the name of the protein is GmMASB1, and the protein can be the protein with the amino acid sequence being a sequence 2 in a sequence table. The gene GmMASB1 is cloned from the soybean variety Qihuang 34, and is over-expressed in arabidopsis thaliana to obtain a transgenic GmMASB1 plant, and the flowering period of the transgenic GmMASB1 plant is obviously earlier than that of a non-transgenic wild type, so that the GmMASB1 protein has a flowering promoting effect and can be applied to plant breeding or variety breeding.

The invention discloses an application of a GmLCL3 gene in regulating the photoperiod and flowering time of soybeans and increasing the yield of the soybeans. According to the invention, a GmLCL3 soybean mutant plant is obtained by using a CRISPR / CAS9 technology, and the GmLCL3 gene in soybeans is mutated, so that the growth period of the soybeans can be prolonged, the blooming of the plant is inhibited, and the yield of the soybeans is increased. Therefore, by constructing a GmLCL3 soybean mutant plant, the sensitivity of soybeans to the photoperiod can be changed, the flowering of the soybeans can be delayed, the growth period of the soybeans can be prolonged, and the yield of the soybeans can be increased. The discovery of the invention has very important academic value for further revealing the molecular mechanism of photoperiod regulation and control of soybean flowering, and provides theoretical basis and genetic resources for promoting the breeding of high-yield and high-qualityvarieties of soybeans.

The invention discloses a tulip pollen collection, identification and preservation method and a tulip crossbreeding method. The tulip pollen collection, identification and preservation method comprises the following steps that (1), pollen collection is conducted; (2) vigour identification is conducted on collected pollen samples; (3), pollen with the germination rate greater than 5% and the germination speed greater than 5 is selected and preserved, wherein the storage temperature is -20-5 DEG C. According to the tulip pollen collection, identification and preservation method, by means of rapid vigour identification, the pollen good in vigour is preserved, preservation is conducted at low temperature, the preservation time is long, the pollen can maintain the vigour for a long time, the problem of blooming of flowers at different times for tulip crossbreeding is effectively solved, and the scientific basis is provided for parent selection.

The invention provides a soybean biological clock gene GmFKF2 protein, (1) the protein is composed of amino acid sequences represented in the SEQ ID NO.1; (2) or the protein is derived from the protein mentioned in (1) through substituting, losing or adding one or several amino acids in the amino acid sequence represented in the SEQ ID No.1, and has the same activity as that of the protein mentioned in (1). The invention at the same time provides a gene GmFKF2, which encodes the protein mentioned above, through over-expressing the gene, plant blossom can be prominently promoted, and the growth period can be prolonged. The soybean biological clock gene GmFKF2 and encoding protein thereof can be used to solve the problems of flowering asynchronism in the crossbreeding process, growth period control of various plants, vegetables, fruits, and flowers, photoperiod sensitivity, and species introduction.

The invention belongs to the field of gene engineering, and provides an optical soybean receptor protein GmZTL2 and application of the optical soybean receptor protein GmZTL2. The protein has an amino acid sequence shown as SEQ ID No.1. The invention also provides a gene for coding the protein. The gene has a nucleotide sequence shown as SEQ ID No.2. By expressing the optical soybean receptor protein GmZTL2, flowering of a plant (arabidopsis thaliana) can be obviously inhibited, the flowering time is delayed, and the growth period is prolonged; and the optical soybean receptor protein GmZTL2 can be used for solving the problems of flowering asynchronism in cross breeding, growth period control of various crops, vegetables, fruits and flowers, photoperiod sensitivity and introduction.

The invention relates to the field of genetic engineering and especially to application of the soybean flowering gene GmCOL4 in regulation and control of florescence of plants. Overexpression of GmCOL4 enables flowering of a plant (Arabidopis thaliana) to be inhibited obviously, flowering time to be delayed and a growth period to be prolonged. The invention can be used for overcoming the problem of flowering asynchronism in crossbreeding, the problem of growth period controlling of a variety of crops, vegetable, fruit and flowers, the problem of photoperiod sensitivity and the problem of introduction.

The invention provides a cultivation method for increasing fructification rate of skimmia reeuesiana. The cultivation method comprises the following steps that healthy and strong skimmia reeuesiana plants are selected for standby application, and the male skimmia reeuesiana plant and the female skimmia reeuesiana plant are transferred into a first standard film greenhouse respectively for cultivation; in the last 30 days when the female skimmia reeuesiana plant is cultivated in the first standard film greenhouse, fertilization of the female skimmia reeuesiana plant is stopped; from July to August, the female skimmia reeuesiana plant in the first standard film greenhouse is transferred into a second standard film greenhouse for cultivation, the fertilization of the female skimmia reeuesianaplant is restored, and a phosphorus potassium fertilizer is applied to the female skimmia reeuesiana plant twice at a frequency of one time per week; from September to November and from December to February of the following year, the cultivation conditions of the second standard film greenhouse are adjusted respectively; after both the female skimmia reeuesiana plant and the male skimmia reeuesiana plant bloom, from 9 am to 11 am, pollen of the male skimmia reeuesiana plant is taken and applied to the pistil column head of the female skimmia reeuesiana plant continuously 2-3 times.

The invention belongs to the technical field of gynoecious strain breeding of bitter gourd, and specifically relates to a rapid identification and seed stocking method for a gynoecious plant of the bitter gourd. The rapid identification and seed stocking method for the gynoecious plant of the bitter gourd concretely comprises the following steps: field planting, pruning treatment, stamen-inducingtreatment, gynoecious plant identification, pollination, and seed stocking. With the method provided by the invention, a gynoecious plant material of the bitter gourd can be accurately screened and identified in an early growth and development stage without the need of clearly knowing the generotype genetic regularity of the bitter gourd or obtaining an accurate molecular marker; gynoecious plantidentification and seed stocking are simultaneously carried out; the problem of asynchronous flower seasons due to concentration of hermaphrodite flowers induced by traditional techniques is solved; interference of a false gynoecious plant is excluded; the screening efficiency of the gynoecious plant of the bitter gourd is improved; and the breeding period of the gynoecious plant of the bitter gourd is shortened.

The invention discloses an ultralow-temperature preservation method of moringa oleifera pollen, wherein the method comprises the following steps: (1) collecting flowers with pollen at 9 o'clock to 11o'clock in the morning; (2) putting the flowers in the step (1) into a sealing bag, drying, sealing, pre-freezing, and storing at the temperature of (-80) DEG C-(-200) DEG C for later use; and (3) thawing the flowers obtained in the step (2) to obtain the moringa oleifera pollen. According to the ultralow-temperature preservation method disclosed by the invention, complete moringa oleifera flowerscan be preserved, and the retention time of the pollen activity can be prolonged to 2-3 months from normal 1-2 days, so that the service life of the pollen is prolonged; according to the thawing mode, secondary freezing in cells in the thawing process can be avoided, and damage to a cell membrane system due to permeation impact of water in the thawing process is prevented, so that the ultralow-temperature pollen cryopreservation effect is effectively guaranteed.

The invention discloses a soybean GmFTL3 protein and a soybean GmFTL5 protein which have the amino acid sequence shown in SEQ ID No.1 and the amino acid sequence SEQ ID No.2 respectively or the sequences which are separately obtained through replacing, deleting or adding one or more of amino acid residues and have the same functions. The GmFTL3 and GmFTL5 which are capable of overexpressing soybean flowering genes are used for obviously promoting the flowering of plant (Arabidopsis thaliana), thus the flowering time can be shortened and the growth period can be shortened. The proteins can be used for solving the problem of flowering asynchronism in the hybridization breeding process, the growth period control problems of various crops, vegetable, fruits and flowers, the problem of photoperiod sensitivity and the introduction problem.

The invention provides a preservation method for paternal pollens in the capsicum hybrid production. In the capsicum hybrid production, male parents and female parents are planted in a time staggering and allopatry mode, and the pollens of the male parents, which are concentratedly stored in liquid nitrogen, are transported to female parent planting sites for the usage during pollination. According to the invention, the flowering asynchronism of the male parents and the female parents in the capsicum hybrid production is thoroughly solved, so the simultaneous and sympatric appearance of capsicum at the seed production male parents and the seed production female parents is avoided, and the loss of the parent of the male parents can be effectively prevented; and superfluous pollens in the seed production can be stored, so the stored pollens can be used to cover the shortage when the amount of the pollens of the male parents is not enough.

The invention discloses a method for ultra-low temperature preservation of Moringa oleifera pollen, which comprises the following steps: (1) selecting flowers with pollen from 9 am to 11 am; (2) placing the flowers in step (1) in a sealed bag , dried, sealed, pre-frozen, and then stored at (-80)°C-(-200)°C for future use; (3) thawing the flowers obtained in step (2) to obtain the Moringa oleifera pollen. The cryopreservation method among the present invention can preserve complete Moringa flower, and the maintenance time of pollen vigor can be extended to 2-3 months by normal 1-2 days, prolongs the life-span of pollen; Among the present invention The thawing method can avoid secondary freezing in the cells during the thawing process, and prevent the osmotic shock of water from damaging the cell membrane system during the thawing process, thereby effectively ensuring the effect of ultra-low temperature freezing of pollen.

The invention relates to the technical field of molecular biology, and provides a soybean phytochrome chromophore synthetic gene GmHY2, an encoded protein thereof and functions of the gene and the encoded protein. According to activity levels of the gene and the encoded protein thereof, leaf chlorophyll content and internode length can be regulated, and the gene and the encoded protein can also be used for regulating photoperiod sensitivity of plants, namely regulating flowering time, can be used for solving the problem of flowering asynchronism in cross breeding, and can provide germplasm resources and theoretical support for breeding eurytopic soybean varieties.

The invention relates to a method for collecting and storing tulip pollen. The method is characterized by comprising the following steps: selecting buds getting ready to burst at a big bud stage in the morning, pushing petals aside slightly with hands, collecting anther by using pliers, putting the anther into a plastic culture dish appropriate in size after the anther is collected, and marking the pollen collection date and the variety information on an upper cover of the culture dish; putting the collected pollen into a room together with the culture dish, putting the culture dish with the pollen into a sealed glass box, putting a drying agent on the bottom of the glass box, arranging a meshy clapboard above the drying agent, putting the culture dish with the pollen on the meshy clapboard, and finishing dehydration after 24 hours; finally, taking the dehydrated pollen out, covering the upper cover of the culture dish, and putting the culture dish in a drying box, then sealing the glass drying box, and storing the drying box for later use under the condition of no direct sunlight at room temperature. The method disclosed by the invention is safe and reliable and simple to operate, the space is saved, and the problem that the tulip pollen cannot be stored for a relatively long time under the natural condition, and the flowering asynchronism problem occurring in a tulip breeding process, and the like are solved.

The invention discloses the hydroponics for the encountance of kiwi fruit in flower period and relates to the encountance method in flower period, which comprises the following steps: i.the start time of water planting of the male plant is 45-55 days earlier than the blooming of the female plant; ii. taking the thrifty and disease and pest free branch in the middle or lower part of the yearly male kiwi fruit according to the time in i., the number of plump sprout in each ear is 2-4, the length of the branch is 13-17cm, shearing the low cut fullmouthly, shearing the up cut in the distance of 0.8-1.2cm from the sprout, immersing the ear in the 400 times MS culture medium with the value of the pH 5.6-5.8, with the immersion depth 1 / 2-5 / 6 of the era length, then water planting in the tissue culture room with the temperature between 18 and 25 DEG C, the relative humidity 70-100% and the illumination intensity 1800-2200 LuX, iii. culturing the ear in the clean water 25-35 days later, changing the water regularly, iv. make the fresh male flower encounter with female flower when the kiwi fruit blooms.

The invention discloses a soybean GmCOL5 gene coded protein. The soybean GmCOL5 gene coded protein is a protein 1 composed of an amino acid sequence shown by the formula of SEQ ID NO.2, or a protein 2 which is derived from the protein 1 through replacement, deletion or addition of one or more amino acids of the amino acid sequence shown by the formula of SEQ ID NO.2 and has activity same as the protein 1. The invention also discloses a soybean GmCOL5 gene for coding the soybean GmCOL5 gene coded protein. Through overexpression of the soybean GmCOL5 gene, plant anthesis is obviously promoted; anthesis is brought forward; and a breeding period is shortened. In crossbreeding, the soybean GmCOL5 gene coded protein and the soybean GmCOL5 gene can solve flowering asynchronism problems, breeding period control problems of various crops, vegetables, fruits and flowers, photoperiodic sensitivity problems and introduction problems.

The invention discloses a gladiolus crossbreeding method using a sunlight greenhouse. Seeds are sown within the mid-to-end of August and the first ten-day period of September when the outdoor highest temperature in not higher than 29 DEG C; cold protection, heat preservation and ventilation are carried out after seedball treatment, soil treatment, soil preparation, sowing and hauling are carried out; daily management refers to moisture management, humiture management and topdressing; artificial pollination is carried out after flowering at the last ten-day period of November in the same year; and seeds are mature 5-6 weeks later. The gladiolus crossbreeding method can greatly delay the flowering phase, effectively solves the inconsistent flowering phase problems of different varieties, and simultaneously provides enough artificial pollination time for crossbreeding work, reduces workload because bagging is not needed before and after flowering, the operation of stamen removal is only carried out before pollen sacs break, and bagging is also not needed before and after pollination, and greatly increases maturing rate, wherein the maturing rate is over 80% and each capsule contains over 60 grains. By adopting the sunlight greenhouse for crossbreeding, the sowing time is delayed, and seasons with high temperature, high humidity and abundant rainwater are avoided, fruit setting is favored and insect pest is prevented.

The invention belongs to the field of gene engineering and relates to sesame SiCOL1 and SiCOL2 genes and application thereof. The sesame SiCOL1 gene has a nucleotide sequence shown as SEQ ID No. 1 or a nucleotide sequence which is formed by being substituted with, deleting or adding one or more nucleotides with equal functions. The sesame SiCOL2 gene has a nucleotide sequence shown as SEQ ID No. 2 or a nucleotide sequence which is formed by being substituted with, deleting or adding one or more nucleotides with equal functions. Overexpression of the SiCOL1 and SiCOL2 genes can change a plant growth stage, can promote flowering of a plant and can be used for solving the asynchronous flowering period problem in cross breeding as well as the growth period control problem, the photoperiod-sensitive problem and the introduction problem of various crops, vegetables, fruits and flowers.

The invention discloses a British wheat germplasm utilization method and belongs to the technical field of crop breeding. According to the British wheat germplasm utilization method, British wheat germplasm is sowed into a greenhouse in a staggered-period mode, domestic wheat germplasm is planted into a seed selection nursery according to normal sowing time, and hybridized combination is assembled to obtain hybrid seeds; the obtained hybrid seeds are sowed into the greenhouse in a staggered-period mode to perform second hybridization with the domestic germplasm planted into the seed selection nursery to obtain second-hybrid F1 generation seeds; and the F1 generation seeds are planted into the seed selection nursery to perform breeding. The method disclosed by the invention utilizes staggered-period sowing in the greenhouse to solve the problem that flowering phases of British wheat germplasm and domestic wheat germplasm are in asynchronism, utilizes hybridization and selection for many times, adjusts a breeding step and inducing, planting and harvesting methods and breeding environment in a breeding process, solves the problem that the British wheat germplasm is difficult to breed and use in domestic and solves the problems of maturing periods and plant heights of hybrid generations. When the method disclosed by the invention is utilized, a plurality of super-high-yield, multi-resistance and eurytopic type wheat new varieties (systems) are bred out.

The invention discloses the cuttage method for the meeting of kiwi fruit in flower period and relates to the plant meeting method in flower period, especially to the kiwi fruit meeting method in flower period. The invention comprises the following steps: (i)cuttage time, the start time of cuttage of the male plant is 45-55 days earlier than the flower time of the female plant; (ii) preparing slotter; (iii) cutting the cuttage-ear; (iv) the management after the cuttage, removing the top when the new ear grows out; (v) making the fresh male flower meet with that of female when the kiwi fruit blooms. The invention possesses the following characteristics: the large quantity of pollen, which guarantees enough pollen supply; the change of flower period, the meeting in the flower period when the flower period varies widely can be realized by controlling the time artificially; the wide range of the use, it can be used in many basal kiwi fruit production area and can also be used to solve the problem of no-meeting in the flower period of other plants.

The invention discloses a method for improving the hybrid pollination and seed setting rate of white konjac and flower konjac, which comprises the following steps: (1) collecting the pollen of white konjac by artificial pollination in an illumination incubator; (2) Preserve the collected white konjac pollen by cryopreservation; (3) revive the cryopreserved white konjac pollen; (4) select flower konjac with fully mature pistil and recovered white konjac pollen for artificial pollination and bagging treatment , when the color of the ovary starts to turn green, the bagging process can be removed. Compared with the prior art, the beneficial effect of the present application is that the amount of white konjac pollen, the purity of the pollen and the vigor of the pollen are all significantly higher than those collected naturally in the field; The rate of pollination and seed setting was significantly improved.