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Rapid identification and seed stocking method for gynoecious plant of bitter gourd

A technology for balsam pear and female plants is applied in the field of rapid identification of all female plants of balsam pear and its seed preservation, which can solve the problems of unsuccessful seed preservation and loss of resources for a single plant, eliminate the interference of climatic and environmental factors, speed up breeding, and improve screening efficiency. Effect

Active Publication Date: 2018-10-12
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, this method is likely to cause concentrated hermaphrodite flowers to be induced and no normal female flowers to pollinate, resulting in failure to save seeds for a single plant during the flowering period, and the phenomenon of resource loss occurs.

Method used

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  • Rapid identification and seed stocking method for gynoecious plant of bitter gourd
  • Rapid identification and seed stocking method for gynoecious plant of bitter gourd

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Effect test

Embodiment 1

[0023] A method for rapid identification of whole female plants of bitter gourd and a method for saving seeds thereof, comprising the following steps:

[0024] (1) field planting: planting MC39-4-5 as the hybrid F of the female parent 2 ;

[0025] (2) Pruning method: leave 3-4 vines at the low nodes where the bitter gourd has not yet begun to bloom;

[0026] (3) Treatment of male induction: at the first flower bud stage, starting from the first flower bud, more than 5 consecutive plants that are all female flower buds, select the growth points of 2-3 vines to induce males, and mark them, And let the branches and tendrils that have not been treated with male attraction be divided into side branches naturally, without finishing. The male lure method is to immerse the growth point of the bitter gourd branch in the silver nitrate solution for 10 seconds and then take it out. The concentration of the silver nitrate solution is 250mg / L;

[0027] (4) Identification of all female p...

Embodiment 2

[0031] A method for rapid identification of whole female plants of bitter gourd and a method for saving seeds thereof, comprising the following steps:

[0032] (1) field planting: planting MC39-4-5 as the hybrid F of the female parent 2 ;

[0033] (2) Pruning method: leave 3-4 vines at the low nodes where the bitter gourd has not yet begun to bloom;

[0034] (3) Treatment of male induction: at the first flower bud stage, starting from the first flower bud, more than 5 consecutive plants that are all female flower buds, select the growth points of 2-3 vines to induce males, and mark them, And let the branches and tendrils that have not been treated with male attraction be divided into side branches naturally, without finishing. The male lure method is to immerse the growing point of the bitter gourd branch in the silver nitrate solution for 10 seconds and then take it out. The concentration of the silver nitrate solution is 280mg / L;

[0035] (4) Identification of all female ...

Embodiment 3

[0039] A method for rapid identification of whole female plants of bitter gourd and a method for saving seeds thereof, comprising the following steps:

[0040] (1) field planting: planting MC39-4-5 as the hybrid F of the female parent 2 ;

[0041] (2) Pruning method: leave 3-4 vines at the low nodes where the bitter gourd has not yet begun to bloom;

[0042] (3) Treatment of male induction: at the first flower bud stage, starting from the first flower bud, more than 5 consecutive plants that are all female flower buds, select the growth points of 2-3 vines to induce males, and mark them, And let the branches and tendrils that have not been treated with male attraction be divided into side branches naturally, without finishing. The male lure method is to immerse the growing point of the bitter gourd branch in the silver nitrate solution for 10 seconds and then take it out. The concentration of the silver nitrate solution is 300mg / L;

[0043] (4) Identification of all female ...

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Abstract

The invention belongs to the technical field of gynoecious strain breeding of bitter gourd, and specifically relates to a rapid identification and seed stocking method for a gynoecious plant of the bitter gourd. The rapid identification and seed stocking method for the gynoecious plant of the bitter gourd concretely comprises the following steps: field planting, pruning treatment, stamen-inducingtreatment, gynoecious plant identification, pollination, and seed stocking. With the method provided by the invention, a gynoecious plant material of the bitter gourd can be accurately screened and identified in an early growth and development stage without the need of clearly knowing the generotype genetic regularity of the bitter gourd or obtaining an accurate molecular marker; gynoecious plantidentification and seed stocking are simultaneously carried out; the problem of asynchronous flower seasons due to concentration of hermaphrodite flowers induced by traditional techniques is solved; interference of a false gynoecious plant is excluded; the screening efficiency of the gynoecious plant of the bitter gourd is improved; and the breeding period of the gynoecious plant of the bitter gourd is shortened.

Description

technical field [0001] The invention belongs to the technical field of breeding of all-female lines of bitter gourd, and in particular relates to a method for rapid identification of all-female lines of bitter gourd and a method for saving seeds. Background technique [0002] There are three types of bitter melon flowers: female flowers, male flowers and bisexual flowers. Under natural conditions, according to the combination of different flowers on the same plant, there are several types of bitter melon flowers. The first type is male and female flowers that appear on the same plant at the same time. Different flower type; the second type is the all-female type in which only female flowers appear on the same plant; the third type is the type in which female flowers, male flowers and bisexual flowers appear on the same plant at the same time, but it is very rare; no other sex type bitter melon resources have been seen so far reports. The dioecious type of bitter gourd is th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04A01G22/05
CPCA01H1/02A01H1/04A01G22/05
Inventor 陈小凤黄如葵黄熊娟冯诚诚黄玉辉梁家作刘杏连秦健琚茜茜
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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