A preparation method of gladiolus nutrient fertilizer, which is composed of: neem leaf powder, garlic powder, tea dry powder, tripterygium powder, dried duck manure, dried rabbit manure, rape straw powder, chicken blood powder, duck blood powder, sorghum cake flour, cottonseed husk powder, sesame cake powder, sunflower seed powder, buckwheat husk powder, bone meal, multivitamins, pine needle powder, mother-of-pearl powder, the percentages described are percentages by weight; various nutrients contained in the formula promote The grown gladiolus leaves are fat, thick and tender, densely growing, strong in growth, good in light reception, rich in color in the corolla, tall and straight inflorescences, like burning sparks, bright and eye-catching, and has excellent landscape effects.