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Multifunctional compound biofertilizer and application thereof

A compound microbial bacteria, multi-functional technology, applied in the application, fertilizer mixture, organic fertilizer and other directions, can solve the problems of lack of good interaction, unreasonable combination of components, low number of viable bacteria in the product, etc., to reduce diseases. Occurrence, good growth promotion effect, and the effect of improving soil fertilizer efficiency

Inactive Publication Date: 2019-04-05
HUBEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing microbial fertilizers have a variety of problems such as single strains, low number of viable bacteria in the product, unreasonable composition of components, and poor interaction between the components, resulting in slow onset of fertilizer effect and insignificant fertilizer effect; therefore , it has become a top priority to develop a compound microbial fertilizer to improve the effect of increasing production

Method used

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  • Multifunctional compound biofertilizer and application thereof
  • Multifunctional compound biofertilizer and application thereof
  • Multifunctional compound biofertilizer and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Preparation of multifunctional compound microbial fertilizer

[0046] A multi-functional compound microbial fertilizer, comprising: fermentation liquid of Burkholderia gladiolus, Lahnella water and nitrogen-fixing bacteria. The fermentation bacteria liquid is as follows: Burkholderia gladiolus, Lahnella water and nitrogen-fixing bacteria were respectively inoculated in beef extract peptone liquid medium for activation and culture for 48 hours, and the number of viable bacteria reached 0.2×10 9 Finally, each microorganism was mixed together at an inoculum amount of 2%, and then inoculated in beef extract peptone medium for 48 hours. After the strain was stabilized, it became a multifunctional composite microbial fertilizer, which was named MEMF03 for subsequent description.

[0047] Described Burkholderia gladiolus is Burkholderia gladiolus MEL01, and its classification name is Burkholderia gladioli MEL01, and this bacterial strain is from the activated sludge of a phosp...

Embodiment 2

[0051] Composite microbial antagonistic effect experiment

[0052] The three strains (MEL01, MEM40, MEM02) activated overnight were streaked in pairs on the LB solid plate, and the growth of the colony at the intersection was observed. The antagonistic effect is shown in figure 1 .

[0053] Depend on figure 1 It can be seen that MEL01, MEM40 and nitrogen-fixing bacteria can grow normally on the plate, and the colony growth at the intersection is normal, and there is no phenomenon of mutual inhibition of bacterial growth; therefore, there is no antagonistic effect between the three strains, and they can be compounded with good results.

Embodiment 3

[0055] Experiment on the Effect of Compound Microorganisms on Decomposing Inorganic Phosphorus

[0056] The overnight activated three strains (MEL01, MEM40, MEM02) and the composite microbial fertilizer MEMF03 were inoculated into PKO medium respectively, and after 24 hours of shaking culture at 28°C and 180rpm, centrifuged at 10000rpm for 8min. Take the supernatant, and measure the soluble phosphorus content according to the national standard method (GB 11893-89-molybdenum antimony anti-colorimetric method), and use the culture medium without inoculation as the control. figure 2 .

[0057] It can be seen from the figure that nitrogen-fixing bacteria have the basic ability to dissolve phosphorus, while MEM40, MEL01 and compound microbial fertilizers have a good dissolving effect on calcium phosphate and phosphate rock powder. Among them, MEM40 can dissolve calcium phosphate and phosphate rock powder respectively. 254.86mg / L, 269.4mg / L, MEL01 can dissolve calcium phosphate an...

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Abstract

The invention discloses a multifunctional compound biofertilizer and belongs to the field of biotechnology. The multifunctional compound biofertilizer comprises fermented liquid of burkholderia gladioli, rahnella aquatilis and azotobacter, having effective functions of phosphorus solubilizing and nitrogen fixation, and can increase effective phosphorus content and nitrogen content needed for growth of plants in soil and improve soil fertility; IAA secreted from the biofertilizer can improve growth of plants; besides, the compound biofertilizer can generate high-activity chitinase, effectivelyinhibit breeding of pathogenic fungus, reduce diseases, further increase and supplement beneficial microorganism of soil and improve status of soil, and thus, the compound biofertilizer has good development prospect in terms of reducing usage of chemical fertilizer, improving soil fertility, improving growth plants and the like.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a multifunctional compound microbial bacterial fertilizer and its application. Background technique [0002] Fertilizer is a strategic material to ensure national food security, and it is the material basis for maintaining and improving soil fertility and realizing sustainable agricultural development. For a long time, in order to rapidly promote crop growth and increase crop yield, a large number of repeated and excessive application of chemical fertilizers and pesticides has caused obvious quality degradation of cultivated land soil in my country, such as loss of aggregate structure, compaction, reduction of organic matter, and soil microbial flora. Imbalance, salinization, etc., resulting in poor crop growth, reduced yield, lower quality, and increased pesticide residues, seriously affecting people's health and safety. Therefore, soil improvement has become a common concern of our...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F11/08C05G3/04C05G3/00C05G3/80
CPCC05F11/08C05G3/00C05G3/80
Inventor 倪红马卫孙孝文张钲佘梦林袁淑博陈晓通
Owner HUBEI UNIV
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